TY - JOUR
T1 - Validation of a point-of-need diagnostic tool for rapid diagnosis of norovirus gastroenteritis
AU - Janapatla, Rajendra Prasad
AU - Lee, Chung Chan
AU - Dudek, Anna
AU - Chuang, Chih Hsien
AU - Chen, Shih Yen
AU - Lai, Chih Ho
AU - Chen, Chyi Liang
AU - Chiu, Cheng Hsun
N1 - Publisher Copyright:
© 2022 Taiwan Pediatric Association
PY - 2022/7
Y1 - 2022/7
N2 - Background: The genogroups GI and GII of norovirus (NoV) ribonucleic acid (RNA) genetic variants are the most prevalent cause of acute gastroenteritis outbreaks, especially in children, worldwide. A fast, accurate and convenient tool for diagnosis of NoV may be preferable to the more complicated performance of real-time reverse transcription-polymerase chain reaction (RT-PCR). Methods: In this study, we developed and evaluated a tool using insulated isothermal PCR (iiPCR)-mediated POCKIT Central NoV GI and NoV GII assay systems for diagnosis of NoV infection in pediatric patients suspected with gastroenteritis. Results: Performance of POCKIT Central Norovirus GI and GII assays using RT-iiPCR, compared to regular real-time RT-PCR showed the same diagnosis rate to NoV GI (100% of total percent agreement and 1.0 of Cohen's kappa value) and a similar detection rate to norovirus GII (96.3% of total percent agreement and 0.92 of Cohen's kappa value). In exclusivity tests, the POCKIT Central NoV GI and GII assays showed negative results to other viruses, indicating that the assays may be a NoV-specific detection tool. Conclusion: POCKIT Central NoV GI and GII Assay systems can provide a simple, rapid, sensitive, and specific point-of-need diagnostic tool for the detection of NoV GI and GII RNAs in clinical specimens from children with acute gastroenteritis.
AB - Background: The genogroups GI and GII of norovirus (NoV) ribonucleic acid (RNA) genetic variants are the most prevalent cause of acute gastroenteritis outbreaks, especially in children, worldwide. A fast, accurate and convenient tool for diagnosis of NoV may be preferable to the more complicated performance of real-time reverse transcription-polymerase chain reaction (RT-PCR). Methods: In this study, we developed and evaluated a tool using insulated isothermal PCR (iiPCR)-mediated POCKIT Central NoV GI and NoV GII assay systems for diagnosis of NoV infection in pediatric patients suspected with gastroenteritis. Results: Performance of POCKIT Central Norovirus GI and GII assays using RT-iiPCR, compared to regular real-time RT-PCR showed the same diagnosis rate to NoV GI (100% of total percent agreement and 1.0 of Cohen's kappa value) and a similar detection rate to norovirus GII (96.3% of total percent agreement and 0.92 of Cohen's kappa value). In exclusivity tests, the POCKIT Central NoV GI and GII assays showed negative results to other viruses, indicating that the assays may be a NoV-specific detection tool. Conclusion: POCKIT Central NoV GI and GII Assay systems can provide a simple, rapid, sensitive, and specific point-of-need diagnostic tool for the detection of NoV GI and GII RNAs in clinical specimens from children with acute gastroenteritis.
KW - Insulated isothermal PCR
KW - Norovirus
KW - Rayleigh-Bénardconvective PCR
UR - http://www.scopus.com/inward/record.url?scp=85128281678&partnerID=8YFLogxK
U2 - 10.1016/j.pedneo.2022.01.003
DO - 10.1016/j.pedneo.2022.01.003
M3 - 文章
C2 - 35379592
AN - SCOPUS:85128281678
SN - 1875-9572
VL - 63
SP - 368
EP - 372
JO - Pediatrics and Neonatology
JF - Pediatrics and Neonatology
IS - 4
ER -