Vibrio salinus sp. nov., a marine nitrogen-fixing bacterium isolated from the lagoon sediment of an islet inside an atoll in the western Pacific Ocean

Wei Sheng Huang, Li Ting Wang, Jwo Sheng Chen, Yen Ting Chen, Sean Ting Shyang Wei, Yin Ru Chiang, Pei Ling Wang, Tzong Huei Lee, Shih Ting Lin, Lina Huang, Wung Yang Shieh*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

Abstract

A marine, facultatively anaerobic, nitrogen-fixing bacterium, designated strain DNF-1T, was isolated from the lagoon sediment of Dongsha Island, Taiwan. Cells grown in broth cultures were Gram-negative rods that were motile by means of monotrichous flagella. Cells grown on plate medium produced prosthecae and vesicle-like structures. NaCl was required and optimal growth occurred at about 2–3% NaCl, 25–30 °C and pH 7–8. The strain grew aerobically and was capable of anaerobic growth by fermenting D-glucose or other carbohydrates as substrate. Both the aerobic and anaerobic growth could be achieved with NH4Cl as a sole nitrogen source. When N2 served as the sole nitrogen source only anaerobic growth was observed. Major cellular fatty acids were C14:0, C16:0 and C16:1ω7c, while major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was 42.2 mol% based on the genomic DNA data. Phylogenetic analyses based on 16S rRNA genes and the housekeeping genes, gapA, pyrH, recA and gyrB, revealed that the strain formed a distinct lineage at species level in the genus Vibrio of the family Vibrionaceae. These results and those from genomic, chemotaxonomic and physiological studies strongly support the assignment of a novel Vibrio species. The name Vibrio salinus sp. nov. is proposed for the novel species, with DNF-1T (= BCRC 81209T = JCM 33626T) as the type strain. This newly proposed species represents the second example of the genus Vibrio that has been demonstrated to be capable of anaerobic growth by fixing N2 as the sole nitrogen source.

Original languageEnglish
Pages (from-to)1203-1214
Number of pages12
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume115
Issue number9
DOIs
StatePublished - 09 2022
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Switzerland AG.

Keywords

  • Marine bacteria
  • Multilocus sequence analysis (MLSA)
  • Nitrogen-fixing bacteria
  • Vibrio
  • Vibrio salinus
  • Vibrionaceae

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