Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen

Maria Duk; Albert M. Wu; Elwira Lisowska

doi:10.1007/BF00731211

Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen

Maria Duk, Albert M. Wu, Elwira Lisowska^*

^*Corresponding author for this work

Department of Molecular Biology

Research output: Contribution to journal › Journal Article › peer-review

5 Scopus citations

Abstract

Earlier studies showed that Moluccella laevis lectin, which has anti-Tn specificity, reacts more strongly with native or desialylated blood group N glycophorin A than with the respective glycophorins of blood group M. We now present results indicating that Vicia villosa B4 anti-Tn lectin, which does not show detectable reaction with untreated glycophorins or erythrocytes, reacts better with desialylated blood group N antigen than with asialo M antigen. This was demonstrated by three assays: (1) agglutination of asialoerythrocytes; (2) binding of biotinylated lectin to asialoerythrocytes immobilized on ELISA plates; and (3) inhibition of lectin binding to asialo-agalactoglycophorin with asialoglycophorins M and N. These results supply further support for the conclusion that glycophorin of blood group N has more GalNAc residues unsubstituted with Gal (Tn receptors) than glycophorin of blood group M.

Original language	English
Pages (from-to)	371-374
Number of pages	4
Journal	Glycoconjugate Journal
Volume	11
Issue number	4
DOIs	https://doi.org/10.1007/BF00731211
State	Published - 08 1994

Keywords

Tn antigen
Vicia villosa lectin
blood groups MN
glycophorin
lectins

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10.1007/BF00731211

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title = "Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen",

abstract = "Earlier studies showed that Moluccella laevis lectin, which has anti-Tn specificity, reacts more strongly with native or desialylated blood group N glycophorin A than with the respective glycophorins of blood group M. We now present results indicating that Vicia villosa B4 anti-Tn lectin, which does not show detectable reaction with untreated glycophorins or erythrocytes, reacts better with desialylated blood group N antigen than with asialo M antigen. This was demonstrated by three assays: (1) agglutination of asialoerythrocytes; (2) binding of biotinylated lectin to asialoerythrocytes immobilized on ELISA plates; and (3) inhibition of lectin binding to asialo-agalactoglycophorin with asialoglycophorins M and N. These results supply further support for the conclusion that glycophorin of blood group N has more GalNAc residues unsubstituted with Gal (Tn receptors) than glycophorin of blood group M.",

keywords = "Tn antigen, Vicia villosa lectin, blood groups MN, glycophorin, lectins",

author = "Maria Duk and Wu, {Albert M.} and Elwira Lisowska",

year = "1994",

month = aug,

doi = "10.1007/BF00731211",

language = "英语",

volume = "11",

pages = "371--374",

journal = "Glycoconjugate Journal",

issn = "0282-0080",

publisher = "Springer",

number = "4",

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TY - JOUR

T1 - Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen

AU - Duk, Maria

AU - Wu, Albert M.

AU - Lisowska, Elwira

PY - 1994/8

Y1 - 1994/8

N2 - Earlier studies showed that Moluccella laevis lectin, which has anti-Tn specificity, reacts more strongly with native or desialylated blood group N glycophorin A than with the respective glycophorins of blood group M. We now present results indicating that Vicia villosa B4 anti-Tn lectin, which does not show detectable reaction with untreated glycophorins or erythrocytes, reacts better with desialylated blood group N antigen than with asialo M antigen. This was demonstrated by three assays: (1) agglutination of asialoerythrocytes; (2) binding of biotinylated lectin to asialoerythrocytes immobilized on ELISA plates; and (3) inhibition of lectin binding to asialo-agalactoglycophorin with asialoglycophorins M and N. These results supply further support for the conclusion that glycophorin of blood group N has more GalNAc residues unsubstituted with Gal (Tn receptors) than glycophorin of blood group M.

AB - Earlier studies showed that Moluccella laevis lectin, which has anti-Tn specificity, reacts more strongly with native or desialylated blood group N glycophorin A than with the respective glycophorins of blood group M. We now present results indicating that Vicia villosa B4 anti-Tn lectin, which does not show detectable reaction with untreated glycophorins or erythrocytes, reacts better with desialylated blood group N antigen than with asialo M antigen. This was demonstrated by three assays: (1) agglutination of asialoerythrocytes; (2) binding of biotinylated lectin to asialoerythrocytes immobilized on ELISA plates; and (3) inhibition of lectin binding to asialo-agalactoglycophorin with asialoglycophorins M and N. These results supply further support for the conclusion that glycophorin of blood group N has more GalNAc residues unsubstituted with Gal (Tn receptors) than glycophorin of blood group M.

KW - Tn antigen

KW - Vicia villosa lectin

KW - blood groups MN

KW - glycophorin

KW - lectins

UR - http://www.scopus.com/inward/record.url?scp=0027946225&partnerID=8YFLogxK

U2 - 10.1007/BF00731211

DO - 10.1007/BF00731211

M3 - 文章

C2 - 7873933

AN - SCOPUS:0027946225

SN - 0282-0080

VL - 11

SP - 371

EP - 374

JO - Glycoconjugate Journal

JF - Glycoconjugate Journal

IS - 4

ER -

Vicia villosa B4 lectin is the second anti-Tn lectin shown to react better with blood group N than M antigen

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