Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43

Hwan You Chang; Jean Hsien Lee; Wen Ling Deng; Tzu Fun Fu; Hwei Ling Peng

doi:10.1006/mpat.1996.0024

Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43

Hwan You Chang, Jean Hsien Lee, Wen Ling Deng, Tzu Fun Fu, Hwei Ling Peng^*

^*Corresponding author for this work

Chang Gung University

Research output: Contribution to journal › Journal Article › peer-review

70 Scopus citations

Abstract

Non-mucoid mutants of a Klebsiella pneumoniae wild type strain CG43 were generated by transposon mutagenesis. One of the mutants was incapable of fermenting galactose and was designated CG43-17. Alterations of the bacterial surface including capsule, lipopolysaccharides, and several species of outer membrane proteins were noted. The mutant was avirulent to mice and became highly sensitive to human serum. The defects could not be complemented by the gaIETK operon. Diminished activity of UDP-glucose pyrophosphorylase in CG43-17 suggested that it is a gaIU mutant. This possibility was confirmed because the parental phenotypes could be fully restored in the mutant by transforming it with a human liver cDNA encoding UDP-glucose pyrophosphorylase.

Original language	English
Pages (from-to)	255-261
Number of pages	7
Journal	Microbial Pathogenesis
Volume	20
Issue number	5
DOIs	https://doi.org/10.1006/mpat.1996.0024
State	Published - 05 1996

Keywords

Klebsiella pneumoniae
gaIU mutant
outer membrane properties

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10.1006/mpat.1996.0024

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title = "Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43",

abstract = "Non-mucoid mutants of a Klebsiella pneumoniae wild type strain CG43 were generated by transposon mutagenesis. One of the mutants was incapable of fermenting galactose and was designated CG43-17. Alterations of the bacterial surface including capsule, lipopolysaccharides, and several species of outer membrane proteins were noted. The mutant was avirulent to mice and became highly sensitive to human serum. The defects could not be complemented by the gaIETK operon. Diminished activity of UDP-glucose pyrophosphorylase in CG43-17 suggested that it is a gaIU mutant. This possibility was confirmed because the parental phenotypes could be fully restored in the mutant by transforming it with a human liver cDNA encoding UDP-glucose pyrophosphorylase.",

keywords = "Klebsiella pneumoniae, gaIU mutant, outer membrane properties",

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T1 - Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43

AU - Chang, Hwan You

AU - Lee, Jean Hsien

AU - Deng, Wen Ling

AU - Fu, Tzu Fun

AU - Peng, Hwei Ling

PY - 1996/5

Y1 - 1996/5

N2 - Non-mucoid mutants of a Klebsiella pneumoniae wild type strain CG43 were generated by transposon mutagenesis. One of the mutants was incapable of fermenting galactose and was designated CG43-17. Alterations of the bacterial surface including capsule, lipopolysaccharides, and several species of outer membrane proteins were noted. The mutant was avirulent to mice and became highly sensitive to human serum. The defects could not be complemented by the gaIETK operon. Diminished activity of UDP-glucose pyrophosphorylase in CG43-17 suggested that it is a gaIU mutant. This possibility was confirmed because the parental phenotypes could be fully restored in the mutant by transforming it with a human liver cDNA encoding UDP-glucose pyrophosphorylase.

AB - Non-mucoid mutants of a Klebsiella pneumoniae wild type strain CG43 were generated by transposon mutagenesis. One of the mutants was incapable of fermenting galactose and was designated CG43-17. Alterations of the bacterial surface including capsule, lipopolysaccharides, and several species of outer membrane proteins were noted. The mutant was avirulent to mice and became highly sensitive to human serum. The defects could not be complemented by the gaIETK operon. Diminished activity of UDP-glucose pyrophosphorylase in CG43-17 suggested that it is a gaIU mutant. This possibility was confirmed because the parental phenotypes could be fully restored in the mutant by transforming it with a human liver cDNA encoding UDP-glucose pyrophosphorylase.

KW - Klebsiella pneumoniae

KW - gaIU mutant

KW - outer membrane properties

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DO - 10.1006/mpat.1996.0024

M3 - 文章

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SN - 0882-4010

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JO - Microbial Pathogenesis

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ER -

Virulence and outer membrane properties of a galU mutant of Klebsiella pneumoniae CG43

Abstract

Keywords

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