Visualization and Quantification of 3D Tumor Cell Migration under Extracellular Stimulation

Andrew Goh, Chun Chih Yeh, Kin Fong Lei*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

9 Scopus citations

Abstract

To investigate tumor cell migration capability, the scratch/wound healing assay and the Transwell assay are the most commonly used assays in the current biomedical research laboratory. However, both assays have their limitations and may mislead the interpretation of the results. In the current study, visualization and quantification of tumor cell migration process was realized in a three-dimensional (3D) environment. The tumor cells horizontally migrated along a Matrigel-filled microchannel under extracellular stimulation. The cell migration process was visualized under a microscope, and the migration speed could be calculated based on the traveling distance of the cells and the time required. Here, three demonstrations were conducted, respectively, including cells attracted by nutrient gradient, stimulated by cytokine, and coculturing with fibroblasts. The results revealed that the cell migration capability could be visually and quantitatively correlated to the extracellular stimulation. The current protocol is compatible to the existing laboratory setup and provides a persuasive result for the study of the 3D cell migration process. Understanding of the molecular and intercellular mechanism of cancer metastasis can potentially develop effective therapeutic strategy.

Original languageEnglish
Pages (from-to)1506-1513
Number of pages8
JournalACS Applied Bio Materials
Volume3
Issue number3
DOIs
StatePublished - 16 03 2020

Bibliographical note

Publisher Copyright:
© 2020 American Chemical Society.

Keywords

  • carcinoma-associated fibroblasts
  • extracellular stimulation
  • microfluidics
  • migration assay
  • tumor cell migration

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