Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice

Ching Hua Hsieh*, Cheng Shyuan Rau, Shao Chun Wu, Johnson Chia Shen Yang, Yi Chan Wu, Tsu Hsiang Lu, Siou Ling Tzeng, Chia Jung Wu, Chia Wei Lin

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review

45 Scopus citations


Background: To examine the circulating microRNA (miRNA) expression profile in a mouse model of diet-induced obesity (DIO) with subsequent weight reduction achieved via low-fat diet (LFD) feeding. Results: Eighteen C57BL/6NCrl male mice were divided into three subgroups: (1) control, mice were fed a standard AIN-76A (fat: 11.5 kcal %) diet for 12 weeks; (2) DIO, mice were fed a 58 kcal % high-fat diet (HFD) for 12 weeks; and (3) DIO + LFD, mice were fed a HFD for 8 weeks to induce obesity and then switched to a 10.5 kcal % LFD for 4 weeks. A switch to LFD feeding led to decreases in body weight, adiposity, and blood glucose levels in DIO mice. Microarray analysis of miRNA using The Mouse & Rat miRNA OneArray® v4 system revealed significant alterations in the expression of miRNAs in DIO and DIO + LFD mice. Notably, 23 circulating miRNAs (mmu-miR-16, mmu-let-7i, mmu-miR-26a, mmu-miR-17, mmu-miR-107, mmu-miR-195, mmu-miR-20a, mmu-miR-25, mmu-miR-15b, mmu-miR-15a, mmu-let-7b, mmu-let-7a, mmu-let-7c, mmu-miR-103, mmu-let-7f, mmu-miR-106a, mmu-miR-106b, mmu-miR-93, mmu-miR-23b, mmu-miR-21, mmu-miR-30b, mmu-miR-221, and mmu-miR-19b) were significantly downregulated in DIO mice but upregulated in DIO + LFD mice. Target prediction and function annotation of associated genes revealed that these genes were predominantly involved in metabolic, insulin signaling, and adipocytokine signaling pathways that directly link the pathophysiological changes associated with obesity and weight reduction. Conclusions: These results imply that obesity-related reductions in the expression of circulating miRNAs could be reversed through changes in metabolism associated with weight reduction achieved through LFD feeding.

Original languageEnglish
Article number699
JournalBMC Genomics
Issue number1
StatePublished - 16 09 2015

Bibliographical note

Publisher Copyright:
© 2015 Hsieh et al.


  • Diet-induced Obesity
  • High-fat Diet
  • Low-fat Diet
  • MicroRNAs


Dive into the research topics of 'Weight-reduction through a low-fat diet causes differential expression of circulating microRNAs in obese C57BL/6 mice'. Together they form a unique fingerprint.

Cite this