TY - JOUR
T1 - Whole blood-derived microRNA signatures in mice exposed to lipopolysaccharides
AU - Hsieh, Ching Hua
AU - Rau, Cheng Shyuan
AU - Jeng, Jonathan Chris
AU - Chen, Yi Chun
AU - Lu, Tsu Hsiang
AU - Wu, Chia Jung
AU - Wu, Yi Chan
AU - Tzeng, Siou Ling
AU - Yang, Johnson Chia Shen
PY - 2012
Y1 - 2012
N2 - Background: Lipopolysaccharide (LPS) is recognized as the most potent microbial mediator presaging the threat of invasion of Gram-negative bacteria that implicated in the pathogenesis of sepsis and septic shock. This study was designed to examine the microRNA (miRNA) expression in whole blood from mice injected with intraperitoneal LPS. Methods: C57BL/6 mice received intraperitoneal injections of varying concentrations (range, 101000 ?g) of LPS from different bacteria, including Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella enterica, and Serratia marcescens and were killed 2, 6, 24, and 72 h after LPS injection. Whole blood samples were obtained and tissues, including lung, brain, liver, and spleen, were harvested for miRNA expression analysis using an miRNA array (Phalanx miRNA OneArray®1.0). Upregulated expression of miRNA targets in the whole blood of C57BL/6 and Tlr4-/-mice injected with LPS was quantified using real-time RT-PCR and compared with that in the whole blood of C57BL/6 mice injected with lipoteichoic acid (LTA) from Staphylococcus aureus. Results: Following LPS injection, a significant increase of 15 miRNAs was observed in the whole blood. Among them, only 3 miRNAs showed up-regulated expression in the lung, but no miRNAs showed a high expression level in the other examined tissues. Upregulated expression of the miRNA targets (let-7d, miR-15b, miR-16, miR-25, miR-92a, miR-103, miR-107 and miR-451) following LPS injection on real-time RT-PCR was dose- and time-dependent. miRNA induction occurred after 2 h and persisted for at least 6 h. Exposure to LPS from different bacteria did not induce significantly different expression of these miRNA targets. Additionally, significantly lower expression levels of let-7d, miR-25, miR-92a, miR-103, and miR-107 were observed in whole blood of Tlr4-/- mice. In contrast, LTA exposure induced moderate expression of miR-451 but not of the other 7 miRNA targets. Conclusions: We identified a specific whole bloodderived miRNA signature in mice exposed to LPS, but not to LTA, from different gram-negative bacteria. These whole blood-derived miRNAs are promising as biomarkers for LPS exposure.
AB - Background: Lipopolysaccharide (LPS) is recognized as the most potent microbial mediator presaging the threat of invasion of Gram-negative bacteria that implicated in the pathogenesis of sepsis and septic shock. This study was designed to examine the microRNA (miRNA) expression in whole blood from mice injected with intraperitoneal LPS. Methods: C57BL/6 mice received intraperitoneal injections of varying concentrations (range, 101000 ?g) of LPS from different bacteria, including Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella enterica, and Serratia marcescens and were killed 2, 6, 24, and 72 h after LPS injection. Whole blood samples were obtained and tissues, including lung, brain, liver, and spleen, were harvested for miRNA expression analysis using an miRNA array (Phalanx miRNA OneArray®1.0). Upregulated expression of miRNA targets in the whole blood of C57BL/6 and Tlr4-/-mice injected with LPS was quantified using real-time RT-PCR and compared with that in the whole blood of C57BL/6 mice injected with lipoteichoic acid (LTA) from Staphylococcus aureus. Results: Following LPS injection, a significant increase of 15 miRNAs was observed in the whole blood. Among them, only 3 miRNAs showed up-regulated expression in the lung, but no miRNAs showed a high expression level in the other examined tissues. Upregulated expression of the miRNA targets (let-7d, miR-15b, miR-16, miR-25, miR-92a, miR-103, miR-107 and miR-451) following LPS injection on real-time RT-PCR was dose- and time-dependent. miRNA induction occurred after 2 h and persisted for at least 6 h. Exposure to LPS from different bacteria did not induce significantly different expression of these miRNA targets. Additionally, significantly lower expression levels of let-7d, miR-25, miR-92a, miR-103, and miR-107 were observed in whole blood of Tlr4-/- mice. In contrast, LTA exposure induced moderate expression of miR-451 but not of the other 7 miRNA targets. Conclusions: We identified a specific whole bloodderived miRNA signature in mice exposed to LPS, but not to LTA, from different gram-negative bacteria. These whole blood-derived miRNAs are promising as biomarkers for LPS exposure.
KW - Gram-negative bacteria
KW - Gram-positive bacteria
KW - Lipopolysaccharide
KW - Lipoteichoic acid
KW - MicroRNAs
KW - Microarray
KW - Toll-like receptor
UR - https://www.scopus.com/pages/publications/84864352443
U2 - 10.1186/1423-0127-19-69
DO - 10.1186/1423-0127-19-69
M3 - 文章
C2 - 22849760
AN - SCOPUS:84864352443
SN - 1021-7770
VL - 19
JO - Journal of Biomedical Science
JF - Journal of Biomedical Science
IS - 1
M1 - 69
ER -
