Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis

Fan Yen Lee; Kuan Hung Chen; Christopher Glenn Wallace; Pei Hsun Sung; Jiunn Jye Sheu; Sheng Ying Chung; Yung Lung Chen; Hung I. Lu; Sheung Fat Ko; Cheuk Kwan Sun; Hsin Ju Chiang; Hsueh Wen Chang; Mel S. Lee; Hon Kan Yip

doi:10.18632/oncotarget.17320

Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis

Fan Yen Lee, Kuan Hung Chen, Christopher Glenn Wallace, Pei Hsun Sung, Jiunn Jye Sheu, Sheng Ying Chung, Yung Lung Chen, Hung I. Lu, Sheung Fat Ko, Cheuk Kwan Sun, Hsin Ju Chiang, Hsueh Wen Chang, Mel S. Lee, Hon Kan Yip^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal Article › peer-review

40 Scopus citations

Abstract

This study tested the hypothesis that xenogeneic human umbilical cord-derived mesenchymal stem cell (HUCDMSC) therapy would improve survival rates in rats with acute respiratory distress-syndrome (ARDS, induction by 48 h inhalation of 100% oxygen) and sepsis-syndrome (SS, induction by cecal-ligation and puncture) (ARDSSS). Adult-male Sprague-Dawley rats were categorized into group 1 (sham-controls), group 2 (ARDS-SS), group 3 [ARDS-SS+HUCDMSC (1.2 x10⁶ cells administered 1 h after SS-induction)], and group 4 [ARDS-SS+HUCDMSC (1.2 x10⁶ cells administered24 h after SS-induction)]. The mortality rate was higher in groups 2 and 4 than in groups 1 and 3 (all p < 0.0001). The blood pressure after 28 h was lower in groups 2, 3 and 4 (p < 0.0001) than in group 1. Albumin levels and percentages of inflammatory cells in broncho-alveolar lavage fluid, and the percentages of inflammatory and immune cells in circulation, were lowest in group 1, highest in group 2, and higher in group 3 than group 4 (all p < 0.0001). The percentages of inflammatory cells in ascites and kidney parenchyma showed identical patterns, as did kidney injury scores (all p < 0.0001). EarlyHUCDMSC therapy reduced rodent mortality after induced ARDS-SS.

Original language	English
Pages (from-to)	45626-45642
Number of pages	17
Journal	Oncotarget
Volume	8
Issue number	28
DOIs	https://doi.org/10.18632/oncotarget.17320
State	Published - 2017

Bibliographical note

Publisher Copyright:
© Lee et al.

Keywords

Inflammatory and immune reactions
Keywords: acute respiratory distress syndrome
Mortality
Sepsis syndrome
Xenogeneic mesenchymal stem cell

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.18632/oncotarget.17320

Cite this

Lee, F. Y., Chen, K. H., Wallace, C. G., Sung, P. H., Sheu, J. J., Chung, S. Y., Chen, Y. L., Lu, H. I., Ko, S. F., Sun, C. K., Chiang, H. J., Chang, H. W., Lee, M. S., & Yip, H. K. (2017). Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis. Oncotarget, 8(28), 45626-45642. https://doi.org/10.18632/oncotarget.17320

@article{425e3d12000f434091efe4a7c6a0356c,

title = "Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis",

abstract = "This study tested the hypothesis that xenogeneic human umbilical cord-derived mesenchymal stem cell (HUCDMSC) therapy would improve survival rates in rats with acute respiratory distress-syndrome (ARDS, induction by 48 h inhalation of 100% oxygen) and sepsis-syndrome (SS, induction by cecal-ligation and puncture) (ARDSSS). Adult-male Sprague-Dawley rats were categorized into group 1 (sham-controls), group 2 (ARDS-SS), group 3 [ARDS-SS+HUCDMSC (1.2 x106 cells administered 1 h after SS-induction)], and group 4 [ARDS-SS+HUCDMSC (1.2 x106 cells administered24 h after SS-induction)]. The mortality rate was higher in groups 2 and 4 than in groups 1 and 3 (all p < 0.0001). The blood pressure after 28 h was lower in groups 2, 3 and 4 (p < 0.0001) than in group 1. Albumin levels and percentages of inflammatory cells in broncho-alveolar lavage fluid, and the percentages of inflammatory and immune cells in circulation, were lowest in group 1, highest in group 2, and higher in group 3 than group 4 (all p < 0.0001). The percentages of inflammatory cells in ascites and kidney parenchyma showed identical patterns, as did kidney injury scores (all p < 0.0001). EarlyHUCDMSC therapy reduced rodent mortality after induced ARDS-SS.",

keywords = "Inflammatory and immune reactions, Keywords: acute respiratory distress syndrome, Mortality, Sepsis syndrome, Xenogeneic mesenchymal stem cell",

author = "Lee, {Fan Yen} and Chen, {Kuan Hung} and Wallace, {Christopher Glenn} and Sung, {Pei Hsun} and Sheu, {Jiunn Jye} and Chung, {Sheng Ying} and Chen, {Yung Lung} and Lu, {Hung I.} and Ko, {Sheung Fat} and Sun, {Cheuk Kwan} and Chiang, {Hsin Ju} and Chang, {Hsueh Wen} and Lee, {Mel S.} and Yip, {Hon Kan}",

note = "Publisher Copyright: {\textcopyright} Lee et al.",

year = "2017",

doi = "10.18632/oncotarget.17320",

language = "英语",

volume = "8",

pages = "45626--45642",

journal = "Oncotarget",

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Lee, FY, Chen, KH, Wallace, CG, Sung, PH, Sheu, JJ, Chung, SY, Chen, YL, Lu, HI, Ko, SF, Sun, CK, Chiang, HJ, Chang, HW, Lee, MS & Yip, HK 2017, 'Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis', Oncotarget, vol. 8, no. 28, pp. 45626-45642. https://doi.org/10.18632/oncotarget.17320

TY - JOUR

T1 - Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis

AU - Lee, Fan Yen

AU - Chen, Kuan Hung

AU - Wallace, Christopher Glenn

AU - Sung, Pei Hsun

AU - Sheu, Jiunn Jye

AU - Chung, Sheng Ying

AU - Chen, Yung Lung

AU - Lu, Hung I.

AU - Ko, Sheung Fat

AU - Sun, Cheuk Kwan

AU - Chiang, Hsin Ju

AU - Chang, Hsueh Wen

AU - Lee, Mel S.

AU - Yip, Hon Kan

PY - 2017

Y1 - 2017

N2 - This study tested the hypothesis that xenogeneic human umbilical cord-derived mesenchymal stem cell (HUCDMSC) therapy would improve survival rates in rats with acute respiratory distress-syndrome (ARDS, induction by 48 h inhalation of 100% oxygen) and sepsis-syndrome (SS, induction by cecal-ligation and puncture) (ARDSSS). Adult-male Sprague-Dawley rats were categorized into group 1 (sham-controls), group 2 (ARDS-SS), group 3 [ARDS-SS+HUCDMSC (1.2 x106 cells administered 1 h after SS-induction)], and group 4 [ARDS-SS+HUCDMSC (1.2 x106 cells administered24 h after SS-induction)]. The mortality rate was higher in groups 2 and 4 than in groups 1 and 3 (all p < 0.0001). The blood pressure after 28 h was lower in groups 2, 3 and 4 (p < 0.0001) than in group 1. Albumin levels and percentages of inflammatory cells in broncho-alveolar lavage fluid, and the percentages of inflammatory and immune cells in circulation, were lowest in group 1, highest in group 2, and higher in group 3 than group 4 (all p < 0.0001). The percentages of inflammatory cells in ascites and kidney parenchyma showed identical patterns, as did kidney injury scores (all p < 0.0001). EarlyHUCDMSC therapy reduced rodent mortality after induced ARDS-SS.

AB - This study tested the hypothesis that xenogeneic human umbilical cord-derived mesenchymal stem cell (HUCDMSC) therapy would improve survival rates in rats with acute respiratory distress-syndrome (ARDS, induction by 48 h inhalation of 100% oxygen) and sepsis-syndrome (SS, induction by cecal-ligation and puncture) (ARDSSS). Adult-male Sprague-Dawley rats were categorized into group 1 (sham-controls), group 2 (ARDS-SS), group 3 [ARDS-SS+HUCDMSC (1.2 x106 cells administered 1 h after SS-induction)], and group 4 [ARDS-SS+HUCDMSC (1.2 x106 cells administered24 h after SS-induction)]. The mortality rate was higher in groups 2 and 4 than in groups 1 and 3 (all p < 0.0001). The blood pressure after 28 h was lower in groups 2, 3 and 4 (p < 0.0001) than in group 1. Albumin levels and percentages of inflammatory cells in broncho-alveolar lavage fluid, and the percentages of inflammatory and immune cells in circulation, were lowest in group 1, highest in group 2, and higher in group 3 than group 4 (all p < 0.0001). The percentages of inflammatory cells in ascites and kidney parenchyma showed identical patterns, as did kidney injury scores (all p < 0.0001). EarlyHUCDMSC therapy reduced rodent mortality after induced ARDS-SS.

KW - Inflammatory and immune reactions

KW - Keywords: acute respiratory distress syndrome

KW - Mortality

KW - Sepsis syndrome

KW - Xenogeneic mesenchymal stem cell

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U2 - 10.18632/oncotarget.17320

DO - 10.18632/oncotarget.17320

M3 - 文章

C2 - 28484089

AN - SCOPUS:85022207350

SN - 1949-2553

VL - 8

SP - 45626

EP - 45642

JO - Oncotarget

JF - Oncotarget

IS - 28

ER -

Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis

Abstract

Bibliographical note

Keywords

UN SDGs

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