YULINK regulates vascular formation in zebrafish and HUVECs

Hsin Hung Lin, Ming Wei Kuo, Tan Chi Fan, Alice L. Yu, John Yu*

*Corresponding author for this work

Research output: Contribution to journalJournal Article peer-review


Background: The distinct arterial and venous cell fates are dictated by a combination of various genetic factors which form diverse types of blood vessels such as arteries, veins, and capillaries. We report here that YULINK protein is involved in vasculogenesis, especially venous formation. Methods: In this manuscript, we employed gene knockdown, yeast two-hybrid, FLIM-FRET, immunoprecipitation, and various imaging technologies to investigate the role of YULINK gene in zebrafish and human umbilical vein endothelial cells (HUVECs). Results: Knockdown of YULINK during the arterial-venous developmental stage of zebrafish embryos led to the defective venous formation and abnormal vascular plexus formation. Knockdown of YULINK in HUVECs impaired their ability to undergo cell migration and differentiation into a capillary-like tube formation. In addition, the phosphorylated EPHB4 was decreased in YULINK knockdown HUVECs. Yeast two-hybrid, FLIM-FRET, immunoprecipitation, as well as imaging technologies showed that YULINK colocalized with endosome related proteins (EPS15, RAB33B or TICAM2) and markers (Clathrin and RHOB). VEGF-induced VEGFR2 internalization was also compromised in YULINK knockdown HUVECs, demonstrating to the involvement of YULINK. Conclusion: This study suggests that YULINK regulates vasculogenesis, possibly through endocytosis in zebrafish and HUVECs.

Original languageEnglish
Article number7
Pages (from-to)7
JournalBiological Research
Issue number1
StatePublished - 27 02 2023

Bibliographical note

© 2023. The Author(s).


  • Clathrin
  • EPS15
  • Endocytosis
  • RAB33B
  • RHOB
  • TICAM2
  • VEGF
  • VEGFR2
  • Vasculogenesis
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Zebrafish/genetics
  • Animals
  • Cell Differentiation
  • Saccharomyces cerevisiae
  • Neovascularization, Physiologic
  • Cell Movement


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