@inproceedings{7647d3b3fdb04807b683ec290b4fdf4e,
title = "A high-throughput perfusion-based micro three-dimensional cell culture platform",
abstract = "This study reports a cell culture platform consisting of 48 microbioreactors for high-throughput perfusion three-dimensional (3-D) cell culture-based assays. Its main characteristics are the capability of conducting multiplex backflow-free medium delivery, and the function for both efficient and high-throughput micro-scale 3-D cell culture construct loading. In addition, a micro-scale perfusion 3-D cell culture-based assay was successfully demonstrated using the proposed cell culture chip. The experimental results were also compared with the same evaluation based on conventional static 3-D cell culture with a larger culture scale. It can be concluded that the choice of the cell culture format can bring different assay results. Therefore, because of the inherent natures of miniaturized perfusion 3-D cell culture, the cell culture chip not only can provide stable, well-defined and more biologically-relevant culture environments, but it also features in low consumption of research resource.",
keywords = "3-D cell culture, Microbioreactors, Microfluidics, Micropumps, Perfusion cell culture",
author = "Huang, {Song Bin} and Wu, {Min Hsien} and Wang, {Shih Siou} and Lin, {Chun Che} and Lee, {Gwo Bin}",
year = "2011",
doi = "10.1109/NEMS.2011.6017294",
language = "英语",
isbn = "9781612847757",
series = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",
pages = "57--60",
booktitle = "NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems",
note = "6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2011 ; Conference date: 20-02-2011 Through 23-02-2011",
}