A syringe-vacuum driven microfluidic chip integrated with beads-based ELISA for early detection of bladder cancer

Ying Ju Chen, Yen Heng Lin*, Chao Sung Lai, Yi Ting Chen, Jau Song Yu, Yu Sun Chang

*此作品的通信作者

研究成果: 圖書/報告稿件的類型會議稿件同行評審

1 引文 斯高帕斯(Scopus)

摘要

This paper reports a negative-pressure driven microfluidic chip, which is capable of rapidly detecting bladder cancer bi-omarker, APOA1, with aid of magnetic beads-based Enzyme-linked immunosorbent assays (ELISA). Fluid can be handled through vacuum force and normally-closed valve. Comparing the experimental results between integrated chip and 96 well plate, the former requires less time (4 hr to 30 min), fewer reagent consumption (100 μl to 30 μl). In this study, the beads-based ELISA in chip has higher detection range and shows better performance in both sensitivity and linearity(99.2%) than conventional ELISA in plate.

原文英語
主出版物標題15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
頁面1692-1694
頁數3
出版狀態已出版 - 2011
事件15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, 美國
持續時間: 02 10 201106 10 2011

出版系列

名字15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
3

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
國家/地區美國
城市Seattle, WA
期間02/10/1106/10/11

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