Amino acid sequence of a specific antigenic peptide of protein B23

P. K. Chan, W. Y. Chan, B. Y.M. Yung, R. G. Cook, M. B. Aldrich, D. Ku, I. L. Goldknopf, H. Busch

研究成果: 期刊稿件文章同行評審

44 引文 斯高帕斯(Scopus)

摘要

A specific antigenic peptide was obtained from protein B23 (M(r)/pI = 37,000/5.1) after 30 min of digestion with staphylococcal V8 protease (10 μg/ml/mg protein B23). The antigenic peptide was purified by DEAE-cellulose chromatography and high pressure liquid chromatography on a reverse-phase C18 column. The antigenic peptide contains 14.7 and 18.7 mol% of glutamic acid and lysine, respectively. Amino acid sequence analysis showed that the peptide has 68 amino acids and is located on the carboxyl-terminal sequence of protein B23. The sequence is Ser-Phe-Lys-Lys-Gln-Glu-Lys-Thr-Pro-Lys-Thr-Pro-Lys-Gly-Pro- Ser-Ser-Val-Glu-Asp-Ile-Lys-Ala-Lys-Met-Gln-ala-Ser-Ile-Glu- Lys-Gly-Gly-Ser-Leu-Pro-Lys-Val-Glu-Ala-Lys-Phe-Ile-Asn-Tyr- Val-Lys-Asn-Cys-Phe-Arg-Met-Thr-Asp-Gln-Glu-Ala-Ile-Gln-Asp- Leu-Trp-Gln-Trp-Arg-Lys-Ser-Leu-COOH. Extensive digestion of the antigenic peptide with V8 protease, trypsin, or chymotrypsin results in loss of the antigenic activity. Three cloned cDNAs (hpB1, hpB2, and hpB7) which code for the 82 amino acids at the COOH terminus of protein B23 and the 3' non-translating sequence were identified and characterized. All three clones have identical nucleotide sequences coding for the antigenic portion of the protein (68 amino acids at the COOH terminus), the stop codon, and the 3' non-translated region. However, mutation of 6 nucleotide bases of one clone (hpB2) caused changes in 4 amino acids in the sequence just preceding the immunoreactive region. The result suggests the presence of at least 2 immunologically similar but distinct proteins which are both recognized by the anti-B23 antibody.

原文英語
頁(從 - 到)14335-14341
頁數7
期刊Journal of Biological Chemistry
261
發行號30
出版狀態已出版 - 1986
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