An integrated microfluidic system for early detection of sepsis-inducing bacteria

Yen Ling Fang, Chih Hung Wang, Yi Sin Chen, Chun Chih Chien, Feng Chih Kuo, Huey Ling You, Mel S. Lee*, Gwo Bin Lee*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

36 引文 斯高帕斯(Scopus)

摘要

Since early diagnosis of sepsis may assist clinicians in initiating timely, effective, and prognosis-improving antibiotic therapy, we developed an integrated microfluidic chip (IMC) for rapid isolation of both Gram-positive and Gram-negative bacteria from blood. The device comprised a membrane-based filtration module (90 min operating time), a bacteria-capturing module using a micro-mixer containing magnetic beads coated with "flexible neck"regions of mannose-binding lectin proteins for bacteria capture (20 min), and a miniature polymerase chain reaction (PCR) module for bacteria identification (90 min via TaqMan® probe technology). The filter separated all white blood cells and 99.5% of red blood cells from bacteria, which were captured at rates approaching 85%. The PCR assay's limit of detection was 5 colony-forming units (CFU) per reaction, and the entire process was completed in only 4 h. Since this is far less than that for culture-based approaches, this IMC may serve as a promising device for detection of sepsis.

原文英語
頁(從 - 到)113-121
頁數9
期刊Lab on a Chip
21
發行號1
DOIs
出版狀態已出版 - 07 01 2021

文獻附註

Publisher Copyright:
© The Royal Society of Chemistry.

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