Analysis of Bioaffinity Sensors for Protein Detection

研究成果: 期刊稿件文章同行評審

摘要

利用數學模式可分析競爭置換原理所製備的親合性感測器。如考慮分析蛋白質及感應蛋白質同時與固定化受體的競爭結合,可導出一三次代數方程式,由其解得到理論檢量線。經由模擬結果可得到設計此型感測器時,幾個需要考慮的因素,以發揮感測器的最大功效。為證實此模式的正確,使用以酸性蛋白質分解酵素及其抑制劑所構成的系統進行實驗,所得結果與理論所預測者吻合。另由此系統動力學方面的探討,也討論了受體與蛋白質間結合強度對感應器應答時間之影響。
Bioaffinity sensors with detection principle of competitive displacement of a responsive protein from an immobilized receptor by an analyte were subject to mathematical analysis. The model considered two competing binding reactions between the analyte and the responsive protein for the immobilized receptor in the solid phase. A cubic algebraic equation generated from the analysis was solved to get theoretical calibration curves for the sensor relating the signal generated, which is the bulk concentration of the responsive protein detected in solution, to the analyte concentration. The simulated results indicated several important system variables to consider when optimizing the sensor’s performance. They are the concentration of the responsive protein initially loaded, the density of the immobilized receptor, and the relative binding strength of both the analyte and the responsive protein toward the receptor. To test this model, experiments were carried out with a hypothetical sensor system, using bovine chymosin as the responsive protein, pepstatin-agarose as the immobilized receptor, and pepsin as the analyte. The model in general can describe the behavior of this type of bioaffinity sensor reasonably well. Form kinetic study of chymosin displacement in the model system, the effect of binding strength between responsive protein and receptor on the response of the sensor was also discussed.
原文美式英語
頁(從 - 到)61-70
期刊Journal of the Chinese Institute of Chemical Engineers
25
發行號1
出版狀態已出版 - 1994

Keywords

  • Acid proteases
  • Bioaffinity
  • Modeling
  • Sensor

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