Anti-sense expression of a metallopeptidase gene enhances nuclear entry of HBV-DNA

Chau Ting Yeh, Hsin Yu Lai, Shiou Ping Chu, I. Chu Tseng

研究成果: 期刊稿件文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Although several putative hepatitis B virus (HBV) receptors have been identified, none of them is capable of initiating HBV replication in a non-permissive human cell line. Using an Epstein-Barr virus-based extrachromosomal replication system, we have screened through a human liver cDNA library and successfully identified a clone capable of facilitating nuclear transport of HBV-DNA during the early phase of HBV infection. This clone contained a cDNA encoding a metallopeptidase-like protein in anti-sense orientation. Pretreatment of naïve HepG2 cells with 1,10-phenanthroline, an inhibitor for liver metallopeptidases, led to nuclear entry of HBV-DNA after HBV infection. However, cccDNA was still undetectable in the nuclei, indicating other cellular factors required to complete the replication cycle were still missing. Our present data suggest that in the initial stage of HBV infection, liver metallopeptidase constitutes a barrier for effective nuclear entry of HBV genomic DNA. Attenuation of metallopeptidase activity may facilitate HBV infection.

原文英語
頁(從 - 到)32-37
頁數6
期刊Biochemical and Biophysical Research Communications
323
發行號1
DOIs
出版狀態已出版 - 08 10 2004
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