Calcitriol exerts a mineralization-inductive effect comparable to that of vitamin C in cultured human periodontium cells

Hsiang Hsi Hong, Adrienne Hong, Chun Chieh Wang, E. Wen Huang, Cheng Cheng Chiang, Tzung Hai Yen*, Yi Fang Huang*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

10 引文 斯高帕斯(Scopus)

摘要

This study inspected whether calcitriol could exert a mineralization-inductive effect comparable to that of vitamin C in cultured human periodontium cells (hPDCs). The mRNA expression of the mineralization-related biomarkers core-binding factor subunit alpha-1 (Cbfa1), collagen 1 α1 (Col-I), alkaline phosphatase (ALP), osteopontin (OPN), bone sialoprotein (BSP), osteocalcin (OCN), vitamin D receptor (VDR), cementum protein 1 (CEMP-1), cementum attachment protein (CAP), interleukin 6 (IL-6), transforming growth factor-β1 (TGF-β1) and osteoprotegerin (OPG) was surveyed after incubation of hPDCs with vitamin C and calcitriol for 2 weeks. Translational expression information from ALP activity and CEMP-1 and CAP immunofluorescence assays was acquired from hPDCs at the second and third weeks. Extracellular calcifications were confirmed by von Kossa staining, Alizarin Red staining and synchrotron transmission X-ray microscopy (TXM) at the fourth and fifth weeks. It was found that both vitamin C and calcitriol not only increased mineralization-related mRNA fold-changes but also enhanced ALP activity, CEMP-1 immunofluorescence, von Kossa and Alizarin Red staining and TXM-associated calcifications. Generally, 10-8M calcitriol displayed greater mineralization significance than 10-7M calcitriol in the assays tested. However, vitamin C stimulated lower Cbfa1, Col-1, ALP, OPN, BSP, OCN, VDR, CEMP-1 and IL-6 mRNA fold-changes than 10-8M calcitriol. Finally, TXM analysis indicated that a 10-8M calcitriol treatment stimulated greater calcifications than vitamin C treatment. Therefore, the analytical results confirmed the osteo-inductive potential of vitamin C in cultured hPDCs. In contrast, 10-8M calcitriol could potentially function as a substitute because it stimulates a greater mineralization effect than vitamin C or 10-7M calcitriol.

原文英語
頁(從 - 到)2304-2316
頁數13
期刊American Journal of Translational Research
11
發行號4
出版狀態已出版 - 2019
對外發佈

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© 2019 E-Century Publishing Corporation. All rights reserved.

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