cDNA cloning of pig testicular lactate dehydrogenase-C, thermal stability of the expressed enzyme, and polymorphism among strains

H. W. Huang; T. Z. Liu; K. H. Lee; C. F. Tu; W. C. Lee; T. Shimogiri; H. Mannen; S. S.L. Li

doi:10.1016/S0378-1119(99)00532-6

cDNA cloning of pig testicular lactate dehydrogenase-C, thermal stability of the expressed enzyme, and polymorphism among strains

H. W. Huang, T. Z. Liu, K. H. Lee, C. F. Tu, W. C. Lee, T. Shimogiri, H. Mannen, S. S.L. Li^*

^*此作品的通信作者

研究成果: 期刊稿件 › 文章 › 同行評審

1 引文斯高帕斯（Scopus）

摘要

Pig testicular lactate dehydrogenase-C (LDHC) cDNA was cloned and sequenced. The deduced sequence of 332 amino acids from pig LDHC shows 73% and 67% identity with that of pig LDHA (muscle) and LDHB (heart) respectively, whereas pig LDHA and LDHB isozymes shows 74% sequence identity. Pig and mouse LDHC cDNAs were subcloned into bacterial expression vector, and the expressed pig LDHC isozyme was shown to be as thermally stable as mouse LDHC isozyme. Pig genomic DNAs from Chinese Meishan, English Yorkshire, Danish Landrace and American Duroc were shown to exhibit polymorphic sites for restriction enzymes EcoRI, BamHI and PstI. (C) 2000 Elsevier Science B.V. All rights reserved.

原文	英語
頁（從 - 到）	151-154
頁數	4
期刊	Gene
卷	242
發行號	1-2
DOIs	https://doi.org/10.1016/S0378-1119(99)00532-6
出版狀態	已出版 - 25 01 2000
對外發佈	是

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title = "cDNA cloning of pig testicular lactate dehydrogenase-C, thermal stability of the expressed enzyme, and polymorphism among strains",

abstract = "Pig testicular lactate dehydrogenase-C (LDHC) cDNA was cloned and sequenced. The deduced sequence of 332 amino acids from pig LDHC shows 73% and 67% identity with that of pig LDHA (muscle) and LDHB (heart) respectively, whereas pig LDHA and LDHB isozymes shows 74% sequence identity. Pig and mouse LDHC cDNAs were subcloned into bacterial expression vector, and the expressed pig LDHC isozyme was shown to be as thermally stable as mouse LDHC isozyme. Pig genomic DNAs from Chinese Meishan, English Yorkshire, Danish Landrace and American Duroc were shown to exhibit polymorphic sites for restriction enzymes EcoRI, BamHI and PstI. (C) 2000 Elsevier Science B.V. All rights reserved.",

keywords = "Porcine, Protein sequence, Restriction sites, Thermal stability",

author = "Huang, {H. W.} and Liu, {T. Z.} and Lee, {K. H.} and Tu, {C. F.} and Lee, {W. C.} and T. Shimogiri and H. Mannen and Li, {S. S.L.}",

year = "2000",

month = jan,

day = "25",

doi = "10.1016/S0378-1119(99)00532-6",

language = "英语",

volume = "242",

pages = "151--154",

journal = "Gene",

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publisher = "Elsevier B.V.",

number = "1-2",

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TY - JOUR

T1 - cDNA cloning of pig testicular lactate dehydrogenase-C, thermal stability of the expressed enzyme, and polymorphism among strains

AU - Huang, H. W.

AU - Liu, T. Z.

AU - Lee, K. H.

AU - Tu, C. F.

AU - Lee, W. C.

AU - Shimogiri, T.

AU - Mannen, H.

AU - Li, S. S.L.

PY - 2000/1/25

Y1 - 2000/1/25

N2 - Pig testicular lactate dehydrogenase-C (LDHC) cDNA was cloned and sequenced. The deduced sequence of 332 amino acids from pig LDHC shows 73% and 67% identity with that of pig LDHA (muscle) and LDHB (heart) respectively, whereas pig LDHA and LDHB isozymes shows 74% sequence identity. Pig and mouse LDHC cDNAs were subcloned into bacterial expression vector, and the expressed pig LDHC isozyme was shown to be as thermally stable as mouse LDHC isozyme. Pig genomic DNAs from Chinese Meishan, English Yorkshire, Danish Landrace and American Duroc were shown to exhibit polymorphic sites for restriction enzymes EcoRI, BamHI and PstI. (C) 2000 Elsevier Science B.V. All rights reserved.

AB - Pig testicular lactate dehydrogenase-C (LDHC) cDNA was cloned and sequenced. The deduced sequence of 332 amino acids from pig LDHC shows 73% and 67% identity with that of pig LDHA (muscle) and LDHB (heart) respectively, whereas pig LDHA and LDHB isozymes shows 74% sequence identity. Pig and mouse LDHC cDNAs were subcloned into bacterial expression vector, and the expressed pig LDHC isozyme was shown to be as thermally stable as mouse LDHC isozyme. Pig genomic DNAs from Chinese Meishan, English Yorkshire, Danish Landrace and American Duroc were shown to exhibit polymorphic sites for restriction enzymes EcoRI, BamHI and PstI. (C) 2000 Elsevier Science B.V. All rights reserved.

KW - Porcine

KW - Protein sequence

KW - Restriction sites

KW - Thermal stability

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U2 - 10.1016/S0378-1119(99)00532-6

DO - 10.1016/S0378-1119(99)00532-6

M3 - 文章

C2 - 10721707

AN - SCOPUS:0033979176

SN - 0378-1119

VL - 242

SP - 151

EP - 154

JO - Gene

JF - Gene

IS - 1-2

ER -

cDNA cloning of pig testicular lactate dehydrogenase-C, thermal stability of the expressed enzyme, and polymorphism among strains

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