Chitosan hydrogel scaffold reinforced with twisted poly(L lactic acid) aligned microfibrous bundle to mimic tendon extracellular matrix

Regeneration of tendon requires construct that provides necessary structural support closely mimicking the native architecture. To recreate this complex architecture a construct made of heat-treated, twisted poly(L lactic acid) (PLLA) microfibers coated with chitosan gel and surrounded by PLLA micro-fibrous layer was developed. The developed construct characterized using SEM showed the macroporous nature of gel coating around four distinct PLLA twisted fibrous bundle and a thin fiber layer surrounding the construct. FTIR analysis confirmed the presence of PLLA and chitosan construct. Mechanical strength increased with increasing number of strips. Protein adsorption was significantly low on the construct with outer covering that could retard cell adhesion to the outer layer. The developed construct showed good cell attachment and proliferation of tenocytes. These results indicate that the construct would find application for tendon tissue engineering.