Cloning a mutated trp operon for the biosynthesis of an antibiotic agent

Plasmid pCG8 containing a mutated trp operon was constructed and introduced into an Escherichiacoli host for increasing tryptophan biosynthesis. Since tryptophan is a biosynthetic precursor of pyrrolnitrin, the mutated trp operon was inserted into a Pseusdomonas vector pME290 to obtain the plasmid pCG12, and then was introduced into Pseudomonaspyrrocinia for enhancing the pyrrolnitrin synthesis. Data showed that the production of pyrrolnitrin of the transformed strain was five-times greater than that of the parental strain.