摘要
DNA topoisomerase I (Top I) contributes to various important biological functions, and its activity is therefore likely regulated in response to different physiological conditions. Increases in both the synthesis and degradation of Top I were previously shown to accompany phytohemagglutinin stimulation of proliferation in human peripheral T lymphocytes. The mechanism of this degradation of Top I has now been investigated with both in vivo and in vitro assays. The activity of a nuclear protease that specifically degrades Top I was induced in proliferating T lymphocytes. The full-length Top I protein (100 kDa) was sequentially degraded to 97- and 82-kDa fragments both in vivo and in vitro. The initial site of proteolytic cleavage was mapped to the NH2-terminal region of the enzyme. The degradation of Top I in vitro was inhibited by aprotinin or soybean trypsin inhibitor, suggesting that the enzyme responsible is a trypsin-like serine protease. Furthermore, Top I degradation by this protease was Mg2+-dependent. The Top I-specific protease activity induced during T lymphocytes proliferation was not detected in Jurkat (human T cell leukemia) cells and various other tested human cancer cell lines, possibly explaining why the abundance of Top I is increased in tumor cells.
原文 | 英語 |
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頁(從 - 到) | 13109-13117 |
頁數 | 9 |
期刊 | Journal of Biological Chemistry |
卷 | 275 |
發行號 | 17 |
DOIs | |
出版狀態 | 已出版 - 28 04 2000 |
對外發佈 | 是 |