Detection of cells captured with antigens on shear horizontal surface-acoustic-wave sensors

Hsu Chao Hao, Hwan You Chang, Tsung Pao Wang, Da Jeng Yao*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

14 引文 斯高帕斯(Scopus)

摘要

Techniques to separate cells are widely applied in immunology. The technique to separate a specific antigen on a microfluidic platform involves the use of a shear horizontal surface-acoustic-wave (SH-SAW) sensor. With specific antibodies conjugated onto the surface of the SH-SAW sensors, this technique can serve to identify specific cells in bodily fluids. Jurkat cells, used as a target in this work, provide a model of cells in small abundance (1:1000) for isolation and purification with the ultimate goal of targeting even more dilute cells. T cells were separated from a mixed-cell medium on a chip (Jurkat cells/K562 cells, 1/1000). A novel microchamber was developed to capture cells during the purification, which required a large biosample. Cell detection was demonstrated through the performance of genetic identification on the chip.

原文英語
頁(從 - 到)69-76
頁數8
期刊Journal of Laboratory Automation
18
發行號1
DOIs
出版狀態已出版 - 02 2013
對外發佈

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