Development of a specific system for targeting protein to metallophilic macrophages

Philip R. Taylor, Susanne Zamze, Richard J. Stillion, Simon Y.C. Wong, Siamon Gordon, Luisa Martinez-Pomares*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

39 引文 斯高帕斯(Scopus)

摘要

The cysteine-rich domain (CR) of the mannose receptor binds sulfated glycoprotein CR ligand (CRL) expressed by subpopulations of myeloid cells in secondary lymphoid organs (CRL+ cells). In naïve mice, these CRL+ cells, metallophilic macrophages (Mφ) in spleen and subcapsular sinus Mφ in lymph nodes, are located strategically for antigen capture and are adjacent to B cell follicles, but their role in the immune response is unknown. We have exploited the lectin activity of CR to develop a highly specific system for targeting protein to CRL+ Mφ. We demonstrate that the sulfated carbohydrates recognized by CR are exposed to the extracellular milieu and mediate highly specific targeting of CR-containing proteins. This model will allow the dissection of the role of metallophilic Mφ in an immune response in vivo.

原文英語
頁(從 - 到)1963-1968
頁數6
期刊Proceedings of the National Academy of Sciences of the United States of America
101
發行號7
DOIs
出版狀態已出版 - 17 02 2004
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