Dynamic embryo culture on a digital microfluidic chip

Chun Chao Li, Yao Wen Hsu, Hong Yuan Huang, Shih Kang Fan

研究成果: 圖書/報告稿件的類型會議稿件同行評審

3 引文 斯高帕斯(Scopus)

摘要

Embryo culture on a digital microfluidic Chip is presented. The digital microfluidic chip consists of two parallel glass plates with conductive., dielectric., and hydrophobic coatings. ICR mouse embryos are cultivated in the culture media (KSOM AA) droplets placed in a petri dish for static studies or between the parallel glass plates for dynamic studies. Embryo culture is successful with the conductive ITO., dielectric SU-8 2002., hydrophobic Teflon layers., and surfactant Pluronic F127 used in the digital microfluidic devices. Moreover., the blastocyst rate of the embryos cultivated in encapsulated droplets containing culture media core droplets and appropriate culture oil shells is comparable to that of the embryos cultivated in traditional petri dishes. Electrowetting-on-dielectric (EWOD) effectively actuates the investigated encapsulated droplets that will be used for dynamic embryo culture.

原文英語
主出版物標題NANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
頁面74-77
頁數4
DOIs
出版狀態已出版 - 2012
對外發佈
事件6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, 泰國
持續時間: 04 11 201207 11 2012

出版系列

名字IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN(列印)2159-6964
ISSN(電子)2159-6972

Conference

Conference6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
國家/地區泰國
城市Bangkok
期間04/11/1207/11/12

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