Effects of Antrodia camphorata extracts on the viability, apoptosis, [Ca 2+ ] i , and MAPKs phosphorylation of OC2 human oral cancer cells

Chorng Chih Huang, He Hsiung Cheng, Jue Long Wang, Jin Shiung Cheng, Kuo Liang Chai, Yi Chien Fang, Chun Chi Kuo, Sau Tung Chu, Chin Man Ho, Ko Liang Lin, Jeng Yu Tsai, Chung Ren Jan*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

12 引文 斯高帕斯(Scopus)

摘要

The effect of Antrodia camphorata (AC) on human oral cancer cells has not been explored. This study examined the effect of AC on the viability, apoptosis, mitogen-activated protein kinases (MAPKs) phosphorylation and Ca 2+ regulation of OC2 human oral cancer cells. AC at a concentration of 25 μM induced an increase in cell viability, but AC at concentrations ≥ 50 μg/ml decreased viability in a concentration-dependent manner. AC at concentrations of 100-200 μg/ml induced apoptosis in a concentration-dependent manner as demonstrated by propidium iodide staining. AC (25 μg/ml) did not alter basal [Ca 2+ ] i , but decreased the [Ca 2+ ] i increases induced by ATP, bradykinin, histamine and thapsigargin. ATP, bradykinin, and histamine increased cell viability whereas thapsigargin decreased it. AC (25 μg/ml) pretreatment failed to alter ATP-induced increase in viability, potentiated bradykinin-induced increase in viability, decreased histamine-induced increase in viability and reversed thapsigargin-induced decrease in viability. Immunoblotting suggested that AC induced phosphorylation of ERK and JNK MAPKs, but not p38 MAPK. Collectively, for OC2 cells, AC exerted multiple effects on their viability and [Ca 2+ ] i , induced their ERK and JNK MAPK phosphorylation, and probably evoked their apoptosis.

原文英語
頁(從 - 到)128-135
頁數8
期刊Chinese Journal of Physiology
52
發行號3
DOIs
出版狀態已出版 - 2009
對外發佈

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