Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field

Hsieh Fu Tsai, Shih Wei Peng, Chun Ying Wu, Hui Fang Chang, Ji Yen Cheng*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

26 引文 斯高帕斯(Scopus)

摘要

We report a new design of microfluidic chip (Multiple electric Field with Uniform Flow chip, MFUF chip) to create multiple electric field strengths (EFSs) while providing a uniform flow field simultaneously. MFUF chip was fabricated from poly-methyl methacrylates (PMMA) substrates by using CO2 laser micromachining. A microfluidic network with interconnecting segments was utilized to de-couple the flow field and the electric field (EF). Using our special design, different EFSs were obtained in channel segments that had an identical cross-section and therefore a uniform flow field. Four electric fields with EFS ratio of 7.9:2.8:1:0 were obtained with flow velocity variation of only 7.8% CV (coefficient of variation). Possible biological effect of shear force can therefore be avoided. Cell behavior under three EFSs and the control condition, where there is no EF, was observed in a single experiment. We validated MFUF chip performance using lung adenocarcinoma cell lines and then used the chip to study the electrotaxis of HSC-3, an oral squamous cell carcinoma cell line. The MFUF chip has high throughput capability for studying the EF-induced cell behavior under various EFSs, including the control condition (EFS = 0).

原文英語
文章編號034116
期刊Biomicrofluidics
6
發行號3
DOIs
出版狀態已出版 - 18 07 2012
對外發佈

指紋

深入研究「Electrotaxis of oral squamous cell carcinoma cells in a multiple-electric-field chip with uniform flow field」主題。共同形成了獨特的指紋。

引用此