Enterovirus D68 vRNA induces type III IFN production via MDA5

Chi Chong Chio, Hio Wai Chan, Shih Hsiang Chen, Hsing I. Huang*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

摘要

Enterovirus D68 (EV-D68) primarily spreads through the respiratory tract and causes respiratory symptoms in children and acute flaccid myelitis (AFM). Type III interferons (IFNs) play a critical role in inhibiting viral growth in respiratory epithelial cells. However, the mechanism by which EV-D68 induces type III IFN production is not yet fully understood. In this study, we show that EV-D68 infection stimulates Calu-3 cells to secrete IFN-λ. The transfection of EV-D68 viral RNA (vRNA) stimulated IFN-λ via MDA5. Furthermore, our findings provide evidence that EV-D68 infection also induces MDA5- IRF3/IRF7-mediated IFN-λ. In addition, we discovered that EV-D68 infection downregulated MDA5 expression. Knockdown of MDA5 increased EV-D68 replication in Calu-3 cells. Finally, we demonstrated that the IFN-λ1 and IFN-λ2/3 proteins effectively inhibit EV-D68 infection in respiratory epithelial cells. In summary, our study shows that EV-D68 induces type III IFN production via the activated MDA5-IRF3/IRF7 pathway and that type III IFNs inhibit EV-D68 replication in Calu-3 cells.

原文英語
文章編號199284
頁(從 - 到)199284
頁數1
期刊Virus Research
339
DOIs
出版狀態已出版 - 02 01 2024

文獻附註

Copyright © 2023. Published by Elsevier B.V.

指紋

深入研究「Enterovirus D68 vRNA induces type III IFN production via MDA5」主題。共同形成了獨特的指紋。

引用此