Evaluating the function of matriptase and N-acetyl-glucosaminyltransferase V in prostate cancer metastasis

Ke Hung Tsui, Phei Lang Chang, Tsui Hsia Feng, Li Chuan Chung, Hsin Ching Sung, Horng Heng Juang*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

32 引文 斯高帕斯(Scopus)

摘要

Previous studies have identified a subclone cell line (PC-J) which was isolated from a metastatic human prostate cell line, PC-3. In vitro matrigel invasion assays and xenograft animal studies suggested that matriptase was a putative metastatic gene in human prostate carcinoma cells. Although low metastatic prostate tumor cells, LNCaP, also expressed high levels of matriptase mRNA, gelatin zymography indicated that LNCaP cells had extremely low matriptase activity. Further studies using RT-PCR and lectin blotting assays revealed that the expression of N-acetylglucosaminyltransferase V (MGAT5), a glyco-protein that stabilizes matriptase, was low in LNCaP cells compared to PC-3 and PC-J cells. The transient overexpression of MGAT5 significantly enhanced the activity of matriptase and the invasion ability in the LNCaP cells. Knock-down of MGAT5 in PC-3 cells attenuated the metastatic ability of the cells, as determined by the in vitro invasion assay and the xenograft animal studies. Matriptase and MGAT5 may play important role in the metastasis of prostate cancer.

原文英語
頁(從 - 到)1993-1999
頁數7
期刊Anticancer Research
28
發行號4 A
出版狀態已出版 - 07 2008

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