@article{c0dc78aa51b54e62a0203e44dff4451d,
title = "Extracellular Vesicular Analysis of Glypican 1 mRNA and Protein for Pancreatic Cancer Diagnosis and Prognosis",
abstract = "Detecting pancreatic duct adenocarcinoma (PDAC) in its early stages and predicting late-stage patient prognosis undergoing chemotherapy is challenging. This work shows that the activation of specific oncogenes leads to elevated expression of mRNAs and their corresponding proteins in extracellular vesicles (EVs) circulating in blood. Utilizing an immune lipoplex nanoparticle (ILN) biochip assay, these findings demonstrate that glypican 1 (GPC1) mRNA expression in the exosomes-rich (Exo) EV subpopulation and GPC1 membrane protein (mProtein) expression in the microvesicles-rich (MV) EV subpopulation, particularly the tumor associated microvesicles (tMV), served as a viable biomarker for PDAC. A combined analysis effectively discriminated early-stage PDAC patients from benign pancreatic diseases and healthy donors in sizable clinical from multiple hospitals. Furthermore, among late-stage PDAC patients undergoing chemotherapy, lower GPC1 tMV-mProtein and Exo-mRNA expression before treatment correlated significantly with prolonged overall survival. These findings underscore the potential of vesicular GPC1 expression for early PDAC screenings and chemotherapy prognosis.",
keywords = "Glypican 1 mRNA in exosomes and protein in tumor-associated microvesicles as a dual biomarker, PDAC screening and chemotherapy prognosis, immune lipoplex nanoparticle biochip assay, single extracellular vesicle analysis, Prognosis, Pancreatic Neoplasms/diagnosis, Humans, Glypicans/genetics, Carcinoma, Pancreatic Ductal/diagnosis, Extracellular Vesicles/metabolism, RNA, Messenger/genetics, Biomarkers, Tumor/genetics",
author = "Hong Li and Chiang, {Chi Ling} and Kwak, {Kwang Joo} and Xinyu Wang and Sital Doddi and Ramanathan, {Lakshmi V.} and Cho, {Sun M.} and Hou, {Ya Chin} and Cheng, {Tai Shan} and Xiaokui Mo and Chang, {Yueh Shih} and Chang, {Hui Lan} and Weiming Cheng and Tsai, {Wei Ni} and Nguyen, {Luong T.H.} and Junjie Pan and Yifan Ma and Rima, {Xilal Y.} and Jingjing Zhang and Eduardo Reategui and Chu, {Yeh Shiu} and Chang, {Peter Mu Hsin} and Chang, {Pei Hung} and Huang, {Chi Ying F.} and Wang, {Cheng Hsu} and Shan, {Yan Shen} and Li, {Chung Pin} and Martin Fleisher and Lee, {L. James}",
note = "{\textcopyright} 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.",
year = "2024",
month = mar,
day = "20",
doi = "10.1002/advs.202306373",
language = "英语",
volume = "11",
pages = "e2306373",
journal = "Advanced Science",
issn = "2198-3844",
number = "11",
}
