Feasibility Study of Using Ultrasound Stimulation to Enhancing Blood-Brain Barrier Disruption in a Brain Tumor Model

C. H. Pan, C. Y. Ting, C. Y. Huang, P. Y. Chen, K. C. Wei, H. L. Liu

研究成果: 圖書/報告稿件的類型會議稿件同行評審

摘要

The purpose of this study is to demonstrate the feasibility of using ultrasound induced BBB-disruption technology to enhance brain-permeable area in a brain tumor model for the enhancement of brain-tumor drug delivery. In our system setup, either 28-kHz non-focused ultrasound or 400-kHz focused ultrasound was used. To examine this, SD rats (n = 14) with C-6 Cell been intestinally implanted to regarded as brain-tumor model. The animals were treated 10 days after tumor implant. During the treatment, the animal were anesthesia I.P., and ultrasound contrast agent was administered I.V. before ultrasound energy excitation to enhance the BBB disruption effect. Blue dye was also administered before animal sacrifice for gross observation of the affected region, and H&E staining were performed to evaluate the ultrasound induced brain tissue damage. BBB-disruptive regions were compared between controlled and ultrasoundtreated animals to see the enhancement of the ultrasound treatment. Results confirmed that ultrasound sonication either 28-kHz or 400-kHz sonication can enhance the BBBdisruption. The pressure threshold of BBB disruption has been confirmed to be 2.25 MPa and 1 MPa in 28 kHz and 400 kHz, respectively. In the 28-kHz sonication case, the BBB-disruptive region surrounding the tumor area can be increased up to 17 fold. Also, tumor-implant animals which underwent localized BBB disruption didn't affect the animal survival, which indicates the ultrasound-induced BBB disruption is secure. The ultrasound-induced BBB disruption may have the potential for brain tumor treatment in the sense of increase the vessel permeability surrounding the brain tumor to increase the local drug concentration near brain tumor.

原文英語
主出版物標題13th International Conference on Biomedical Engineering - ICBME 2008
頁面536-539
頁數4
DOIs
出版狀態已出版 - 2009
事件13th International Conference on Biomedical Engineering, ICBME 2008 - , 新加坡
持續時間: 03 12 200806 12 2008

出版系列

名字IFMBE Proceedings
23
ISSN(列印)1680-0737

Conference

Conference13th International Conference on Biomedical Engineering, ICBME 2008
國家/地區新加坡
期間03/12/0806/12/08

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