Fluoroquinolone Resistance in Clinical Isolates of Streptococcus pneumoniae from Asian Countries: ANSORP Study

Won Sup Oh, Ji Yoeun Suh, Jae Hoon Song*, Kwan Soo Ko, Sook In Jung, Kyong Ran Peck, Nam Yong Lee, Yonghong Yang, Anan Chongthaleong, Cheng Hsun Chiu, Adeeba Kamarulzaman, Navartnam Parasakthi, M. K. Lalitha, Jennifer Perera, Ti Teow Yee, Gamini Kumarasinghe, Celia C. Carlos

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

17 引文 斯高帕斯(Scopus)

摘要

Seventeen clinical isolates of Streptococcus pneumoniae showing reduced susceptibility to ciprofloxacin (MIC ≥ 4 μg/ml) collected from eight different Asian countries were analyzed by antimicrobial susceptibility, serotyping, pulsed-field gel electrophoresis (PFGE), and DNA sequencing of the quinolone resistance-determining regions (QRDRs) in gyrA, gyrB, parC, and parE. All isolates but one showed more than one amino acid alteration in QRDRs of four responsible genes. Ile460 → Val in parE was the most common mutation. Data suggest that Lys137 → Asn in parC may be a primary step in the development of high-level and multiple FQ resistance. An additional mutation of Ser81 → Phe in gyrA resulted in high-level resistance to ciprofloxacin, levofloxacin, and gatifloxacin, whereas Ser79 → Phe in parC may exert an important role in the development of moxifloxacin resistance. Two novel amino acid changes in gyrB, Ala390 → Val and Asn423 → Thr, were found. Data from PFGE suggest an introduction and local spread of multiple resistant Spain23F-1 clone in Hong Kong, but isolates from other Asian countries were not related to this clone.

原文英語
頁(從 - 到)37-42
頁數6
期刊Microbial Drug Resistance
10
發行號1
DOIs
出版狀態已出版 - 03 2004
對外發佈

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