Identification of microRNA in the protist Trichomonas vaginalis

Wei Chen Lin; Sung Chou Li; Wen Chang Lin; Jyh Wei Shin; Song Nian Hu; Xiao Min Yu; Ting Yun Huang; Shih Chieh Chen; Hua Chien Chen; Shu Jen Chen; Po Jung Huang; Richie Ruei-Chi Gan; Cheng Hsun Chiu; Petrus Tang

doi:10.1016/j.ygeno.2009.01.004

Identification of microRNA in the protist Trichomonas vaginalis

Wei Chen Lin, Sung Chou Li, Wen Chang Lin, Jyh Wei Shin, Song Nian Hu, Xiao Min Yu, Ting Yun Huang, Shih Chieh Chen, Hua Chien Chen, Shu Jen Chen, Po Jung Huang, Richie Ruei-Chi Gan, Cheng Hsun Chiu, Petrus Tang^*

^*此作品的通信作者

研究成果: 期刊稿件 › 文章 › 同行評審

62 引文斯高帕斯（Scopus）

摘要

MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. However, miRNA has never been identified experimentally in protist. Direct cloning of 438 expressed miRNA tags by microRNA serial analysis of gene expression from the parasitic protist Trichomonas vaginalis identified nine candidate miRNAs. Bioinformatics analysis of the corresponding genomic region revealed that these miRNA candidates contain a classical stem-loop-stem structure of pre-microRNAs. Analysis of the 20 nt long mature tva-miR-001 showed that it is an intergenic miRNA located at the scaffold DS113596. Tva-miR-001 was differentially expressed in the trophozoite, pseudocyst and amoeboid stages. Based on the experimental results of the present study, we provided solid evidence that protist possesses a miRNA regulating network comparable with multicellular organisms for the first time.

原文	英語
頁（從 - 到）	487-493
頁數	7
期刊	Genomics
卷	93
發行號	5
DOIs	https://doi.org/10.1016/j.ygeno.2009.01.004
出版狀態	已出版 - 05 2009

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title = "Identification of microRNA in the protist Trichomonas vaginalis",

abstract = "MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. However, miRNA has never been identified experimentally in protist. Direct cloning of 438 expressed miRNA tags by microRNA serial analysis of gene expression from the parasitic protist Trichomonas vaginalis identified nine candidate miRNAs. Bioinformatics analysis of the corresponding genomic region revealed that these miRNA candidates contain a classical stem-loop-stem structure of pre-microRNAs. Analysis of the 20 nt long mature tva-miR-001 showed that it is an intergenic miRNA located at the scaffold DS113596. Tva-miR-001 was differentially expressed in the trophozoite, pseudocyst and amoeboid stages. Based on the experimental results of the present study, we provided solid evidence that protist possesses a miRNA regulating network comparable with multicellular organisms for the first time.",

keywords = "MicroRNA, Protist, Trichomonas vaginalis",

author = "Lin, {Wei Chen} and Li, {Sung Chou} and Lin, {Wen Chang} and Shin, {Jyh Wei} and Hu, {Song Nian} and Yu, {Xiao Min} and Huang, {Ting Yun} and Chen, {Shih Chieh} and Chen, {Hua Chien} and Chen, {Shu Jen} and Huang, {Po Jung} and {Ruei-Chi Gan}, Richie and Chiu, {Cheng Hsun} and Petrus Tang",

year = "2009",

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doi = "10.1016/j.ygeno.2009.01.004",

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AU - Lin, Wei Chen

AU - Li, Sung Chou

AU - Lin, Wen Chang

AU - Shin, Jyh Wei

AU - Hu, Song Nian

AU - Yu, Xiao Min

AU - Huang, Ting Yun

AU - Chen, Shih Chieh

AU - Chen, Hua Chien

AU - Chen, Shu Jen

AU - Huang, Po Jung

AU - Ruei-Chi Gan, Richie

AU - Chiu, Cheng Hsun

AU - Tang, Petrus

PY - 2009/5

Y1 - 2009/5

N2 - MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. However, miRNA has never been identified experimentally in protist. Direct cloning of 438 expressed miRNA tags by microRNA serial analysis of gene expression from the parasitic protist Trichomonas vaginalis identified nine candidate miRNAs. Bioinformatics analysis of the corresponding genomic region revealed that these miRNA candidates contain a classical stem-loop-stem structure of pre-microRNAs. Analysis of the 20 nt long mature tva-miR-001 showed that it is an intergenic miRNA located at the scaffold DS113596. Tva-miR-001 was differentially expressed in the trophozoite, pseudocyst and amoeboid stages. Based on the experimental results of the present study, we provided solid evidence that protist possesses a miRNA regulating network comparable with multicellular organisms for the first time.

AB - MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. However, miRNA has never been identified experimentally in protist. Direct cloning of 438 expressed miRNA tags by microRNA serial analysis of gene expression from the parasitic protist Trichomonas vaginalis identified nine candidate miRNAs. Bioinformatics analysis of the corresponding genomic region revealed that these miRNA candidates contain a classical stem-loop-stem structure of pre-microRNAs. Analysis of the 20 nt long mature tva-miR-001 showed that it is an intergenic miRNA located at the scaffold DS113596. Tva-miR-001 was differentially expressed in the trophozoite, pseudocyst and amoeboid stages. Based on the experimental results of the present study, we provided solid evidence that protist possesses a miRNA regulating network comparable with multicellular organisms for the first time.

KW - MicroRNA

KW - Protist

KW - Trichomonas vaginalis

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U2 - 10.1016/j.ygeno.2009.01.004

DO - 10.1016/j.ygeno.2009.01.004

M3 - 文章

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AN - SCOPUS:64149117818

SN - 0888-7543

VL - 93

SP - 487

EP - 493

JO - Genomics

JF - Genomics

IS - 5

ER -

Identification of microRNA in the protist Trichomonas vaginalis

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