Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

Jheng Syong Wu; Yung Chin Chung; Jun Jei Chien; Chien Chou

doi:10.1117/1.JBO.23.1.010502

Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

Jheng Syong Wu, Yung Chin Chung, Jun Jei Chien, Chien Chou^*

^*此作品的通信作者

研究成果: 期刊稿件 › 文章 › 同行評審

4 引文斯高帕斯（Scopus）

摘要

A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

原文	英語
文章編號	010502
期刊	Journal of Biomedical Optics
卷	23
發行號	1
DOIs	https://doi.org/10.1117/1.JBO.23.1.010502
出版狀態	已出版 - 01 2018
對外發佈	是

文獻附註

Publisher Copyright:
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).

存取文件

10.1117/1.JBO.23.1.010502

其它文件與鏈接

Link to publication in Scopus

引用此

@article{7a1175993d6b4b65861aeaf066921ae6,

title = "Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope",

abstract = "A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.",

keywords = "aberrations, confocal fluorescence microscopy, optical heterodyne",

author = "Wu, {Jheng Syong} and Chung, {Yung Chin} and Chien, {Jun Jei} and Chien Chou",

note = "Publisher Copyright: {\textcopyright} 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).",

year = "2018",

month = jan,

doi = "10.1117/1.JBO.23.1.010502",

language = "英语",

volume = "23",

journal = "Journal of Biomedical Optics",

issn = "1083-3668",

publisher = "SPIE",

number = "1",

}

TY - JOUR

T1 - Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

AU - Wu, Jheng Syong

AU - Chung, Yung Chin

AU - Chien, Jun Jei

AU - Chou, Chien

PY - 2018/1

Y1 - 2018/1

N2 - A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

AB - A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

KW - aberrations

KW - confocal fluorescence microscopy

KW - optical heterodyne

UR - http://www.scopus.com/inward/record.url?scp=85040829620&partnerID=8YFLogxK

U2 - 10.1117/1.JBO.23.1.010502

DO - 10.1117/1.JBO.23.1.010502

M3 - 文章

C2 - 29341543

AN - SCOPUS:85040829620

SN - 1083-3668

VL - 23

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 1

M1 - 010502

ER -

Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

摘要

文獻附註

存取文件

其它文件與鏈接

指紋

引用此