Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

Jheng Syong Wu; Yung Chin Chung; Jun Jei Chien; Chien Chou

doi:10.1117/1.JBO.23.1.010502

Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

Jheng Syong Wu
, Yung Chin Chung
, Jun Jei Chien
, Chien Chou^*

^*此作品的通信作者

Chang Gung University
Academia Sinica - Institute of Chemistry

研究成果: 期刊稿件 › 文章 › 同行評審

4 引文斯高帕斯（Scopus）

摘要

A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

原文	英語
文章編號	010502
期刊	Journal of Biomedical Optics
卷	23
發行號	1
DOIs	https://doi.org/10.1117/1.JBO.23.1.010502
出版狀態	已出版 - 01 2018
對外發佈	是

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Publisher Copyright:
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE).

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10.1117/1.JBO.23.1.010502

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abstract = "A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.",

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author = "Wu, \{Jheng Syong\} and Chung, \{Yung Chin\} and Chien, \{Jun Jei\} and Chien Chou",

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AU - Wu, Jheng Syong

AU - Chung, Yung Chin

AU - Chien, Jun Jei

AU - Chou, Chien

PY - 2018/1

Y1 - 2018/1

N2 - A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

AB - A two-frequency laser scanning confocal fluorescence microscope (TF-LSCFM) based on intensity modulated fluorescence signal detection was proposed. The specimen-induced spherical aberration and scattering effect were suppressed intrinsically, and high image contrast was presented due to heterodyne interference. An improved axial point spread function in a TF-LSCFM compared with a conventional laser scanning confocal fluorescence microscope was demonstrated and discussed.

KW - aberrations

KW - confocal fluorescence microscopy

KW - optical heterodyne

UR - https://www.scopus.com/pages/publications/85040829620

U2 - 10.1117/1.JBO.23.1.010502

DO - 10.1117/1.JBO.23.1.010502

M3 - 文章

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AN - SCOPUS:85040829620

SN - 1083-3668

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JO - Journal of Biomedical Optics

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ER -

Improved axial point spread function in a two-frequency laser scanning confocal fluorescence microscope

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