In vitro dynamic fertilization by using EWOD device

Lung Yuan Chung; Hsien Hua Shen; Yu Hsiang Chung; Chih Chen Chen; Chia Hsien Hsu; Hong Yuan Huang; Da Jeng Yao

doi:10.1109/MEMSYS.2015.7051006

In vitro dynamic fertilization by using EWOD device

Lung Yuan Chung, Hsien Hua Shen, Yu Hsiang Chung, Chih Chen Chen, Chia Hsien Hsu, Hong Yuan Huang, Da Jeng Yao

研究成果: 圖書/報告稿件的類型 › 會議稿件 › 同行評審

摘要

This research demonstrates the in vitro fertilization (IVF) technique by using the digital microfluidic (DMF) system. The DMF device has been proved with biocompatibility and used in this research for mouse gametes in vitro fertilization and embryos culture based on the dispersed droplet form. Dynamically moving, cell culture and observation can be achieved by the time-lapse microscopy in an incubator. The fertilization rate of IVF on DMF is 34.8%, and about 25% inseminated embryos dynamically cultured on DMF chip could develop into 8-cell stage. The result indicated that the DMF system has the potential used for assisted reproductive technology.

原文	英語
主出版物標題	2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015
發行者	Institute of Electrical and Electronics Engineers Inc.
頁面	519-522
頁數	4
版本	February
ISBN（電子）	9781479979554
DOIs	https://doi.org/10.1109/MEMSYS.2015.7051006
出版狀態	已出版 - 26 02 2015
對外發佈	是
事件	2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 - Estoril, 葡萄牙持續時間: 18 01 2015 → 22 01 2015

出版系列

名字	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
號碼	February
卷	2015-February
ISSN（列印）	1084-6999

Conference

Conference	2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015
國家/地區	葡萄牙
城市	Estoril
期間	18/01/15 → 22/01/15

文獻附註

Publisher Copyright:
© 2015 IEEE.

存取文件

10.1109/MEMSYS.2015.7051006

其它文件與鏈接

Link to publication in Scopus

引用此

Chung, L. Y., Shen, H. H., Chung, Y. H., Chen, C. C., Hsu, C. H., Huang, H. Y., & Yao, D. J. (2015). In vitro dynamic fertilization by using EWOD device. 於 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 (February 編輯, 頁 519-522). 文章 7051006 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); 卷 2015-February, 編號 February). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2015.7051006

Chung, Lung Yuan ; Shen, Hsien Hua ; Chung, Yu Hsiang 等. / In vitro dynamic fertilization by using EWOD device. 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015. February. 編輯 Institute of Electrical and Electronics Engineers Inc., 2015. 頁 519-522 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); February).

@inproceedings{f042336f00b04e91a48e2f26c4508f07,

title = "In vitro dynamic fertilization by using EWOD device",

abstract = "This research demonstrates the in vitro fertilization (IVF) technique by using the digital microfluidic (DMF) system. The DMF device has been proved with biocompatibility and used in this research for mouse gametes in vitro fertilization and embryos culture based on the dispersed droplet form. Dynamically moving, cell culture and observation can be achieved by the time-lapse microscopy in an incubator. The fertilization rate of IVF on DMF is 34.8\%, and about 25\% inseminated embryos dynamically cultured on DMF chip could develop into 8-cell stage. The result indicated that the DMF system has the potential used for assisted reproductive technology.",

author = "Chung, \{Lung Yuan\} and Shen, \{Hsien Hua\} and Chung, \{Yu Hsiang\} and Chen, \{Chih Chen\} and Hsu, \{Chia Hsien\} and Huang, \{Hong Yuan\} and Yao, \{Da Jeng\}",

note = "Publisher Copyright: {\textcopyright} 2015 IEEE.; 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 ; Conference date: 18-01-2015 Through 22-01-2015",

year = "2015",

month = feb,

day = "26",

doi = "10.1109/MEMSYS.2015.7051006",

language = "英语",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

number = "February",

pages = "519--522",

booktitle = "2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015",

address = "美国",

edition = "February",

}

Chung, LY, Shen, HH, Chung, YH, Chen, CC, Hsu, CH, Huang, HY & Yao, DJ 2015, In vitro dynamic fertilization by using EWOD device. 於 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015. February edn, 7051006, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 編號 February, 卷 2015-February, Institute of Electrical and Electronics Engineers Inc., 頁 519-522, 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015, Estoril, 葡萄牙, 18/01/15. https://doi.org/10.1109/MEMSYS.2015.7051006

In vitro dynamic fertilization by using EWOD device. / Chung, Lung Yuan; Shen, Hsien Hua; Chung, Yu Hsiang 等.
2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015. February. 編輯 Institute of Electrical and Electronics Engineers Inc., 2015. p. 519-522 7051006 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); 卷 2015-February, 編號 February).

研究成果: 圖書/報告稿件的類型 › 會議稿件 › 同行評審

TY - GEN

T1 - In vitro dynamic fertilization by using EWOD device

AU - Chung, Lung Yuan

AU - Shen, Hsien Hua

AU - Chung, Yu Hsiang

AU - Chen, Chih Chen

AU - Hsu, Chia Hsien

AU - Huang, Hong Yuan

AU - Yao, Da Jeng

PY - 2015/2/26

Y1 - 2015/2/26

N2 - This research demonstrates the in vitro fertilization (IVF) technique by using the digital microfluidic (DMF) system. The DMF device has been proved with biocompatibility and used in this research for mouse gametes in vitro fertilization and embryos culture based on the dispersed droplet form. Dynamically moving, cell culture and observation can be achieved by the time-lapse microscopy in an incubator. The fertilization rate of IVF on DMF is 34.8%, and about 25% inseminated embryos dynamically cultured on DMF chip could develop into 8-cell stage. The result indicated that the DMF system has the potential used for assisted reproductive technology.

AB - This research demonstrates the in vitro fertilization (IVF) technique by using the digital microfluidic (DMF) system. The DMF device has been proved with biocompatibility and used in this research for mouse gametes in vitro fertilization and embryos culture based on the dispersed droplet form. Dynamically moving, cell culture and observation can be achieved by the time-lapse microscopy in an incubator. The fertilization rate of IVF on DMF is 34.8%, and about 25% inseminated embryos dynamically cultured on DMF chip could develop into 8-cell stage. The result indicated that the DMF system has the potential used for assisted reproductive technology.

UR - https://www.scopus.com/pages/publications/84931034468

U2 - 10.1109/MEMSYS.2015.7051006

DO - 10.1109/MEMSYS.2015.7051006

M3 - 会议稿件

AN - SCOPUS:84931034468

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 519

EP - 522

BT - 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015

PB - Institute of Electrical and Electronics Engineers Inc.

T2 - 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015

Y2 - 18 January 2015 through 22 January 2015

ER -

Chung LY, Shen HH, Chung YH, Chen CC, Hsu CH, Huang HY 等. In vitro dynamic fertilization by using EWOD device. 於 2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015. February 編輯 Institute of Electrical and Electronics Engineers Inc. 2015. p. 519-522. 7051006. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); February). doi: 10.1109/MEMSYS.2015.7051006

In vitro dynamic fertilization by using EWOD device

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