TY - GEN
T1 - In vitro experiments on rapid prototyping laser sintered porous PCL scaffolds with polymer hydrogel
AU - Lee, Ming Yih
AU - Liu, Si Wen
AU - Chen, Jyh Ping
AU - Liao, Han Tsung
AU - Tsai, Wen Wei
AU - Wang, Hsiu Chen
AU - Lin, Hung Ming
PY - 2011
Y1 - 2011
N2 - Bone defects caused by tumors, diseased infection, trauma or abnormal bone development create a lot of serious health problems. Tissue engineering aims to fabricate tissues or organs using the cells of patients for repairing the damaged tissues or organs in clinic. The aim of this study was to design and fabricate polycaprolactone (PCL) scaffolds using the inhouse-built selective laser sintering (SLS) rapid prototyping (RP) machine and combine with polymer hydrogel for in vitro study for bone repair. In this study, three configurations of scaffolds structure (0/45/0/45°, 0/90/0/90°, and 0/45/90/135° patterns) were designed and produced. The compressive modulus, porosity and pore size of porous scaffolds were first determined. In addition, polymer hydrogel was combined with PCL scaffolds with three loading methods (i.e. immersion method, injection method and titration method) to enhance scaffolds surface hydrophilicity for cell proliferation. Mesenchymal stem cells from New Zealand White rabbits were loaded to PCL scaffolds and induced to osteoblasts in vitro. Bone formation was determined by MTS assays, von Kossa stains and ALP activities. The experimental results showed the compressive moduli of scaffolds with 0/45/0/45°,0/90/0/90°, and 0/45/90/135° patterns were 2 MPa, 3.4 MPa, and 3.75 MPa, respectively. The porosity of scaffolds was 72%, 76%, and 83%, respectively. The ranges of pore size of scaffolds were 350-400 μm, 400-500 μm, and 350-400 μm, respectively. By comparing three kinds of polymer hydrogel loading methods, titration method had the best result. The in vitro experimental results revealed that OD values of MTS tests and ALP activities increased from day 7 to day 21 and von Kossa stain revealed dark brown mineralized tissue, indicating cells could proliferate and differentiate in polymer hydrogel and scaffolds.
AB - Bone defects caused by tumors, diseased infection, trauma or abnormal bone development create a lot of serious health problems. Tissue engineering aims to fabricate tissues or organs using the cells of patients for repairing the damaged tissues or organs in clinic. The aim of this study was to design and fabricate polycaprolactone (PCL) scaffolds using the inhouse-built selective laser sintering (SLS) rapid prototyping (RP) machine and combine with polymer hydrogel for in vitro study for bone repair. In this study, three configurations of scaffolds structure (0/45/0/45°, 0/90/0/90°, and 0/45/90/135° patterns) were designed and produced. The compressive modulus, porosity and pore size of porous scaffolds were first determined. In addition, polymer hydrogel was combined with PCL scaffolds with three loading methods (i.e. immersion method, injection method and titration method) to enhance scaffolds surface hydrophilicity for cell proliferation. Mesenchymal stem cells from New Zealand White rabbits were loaded to PCL scaffolds and induced to osteoblasts in vitro. Bone formation was determined by MTS assays, von Kossa stains and ALP activities. The experimental results showed the compressive moduli of scaffolds with 0/45/0/45°,0/90/0/90°, and 0/45/90/135° patterns were 2 MPa, 3.4 MPa, and 3.75 MPa, respectively. The porosity of scaffolds was 72%, 76%, and 83%, respectively. The ranges of pore size of scaffolds were 350-400 μm, 400-500 μm, and 350-400 μm, respectively. By comparing three kinds of polymer hydrogel loading methods, titration method had the best result. The in vitro experimental results revealed that OD values of MTS tests and ALP activities increased from day 7 to day 21 and von Kossa stain revealed dark brown mineralized tissue, indicating cells could proliferate and differentiate in polymer hydrogel and scaffolds.
UR - https://www.scopus.com/pages/publications/79952501773
U2 - 10.3850/978-981-08-7615-9-TE02
DO - 10.3850/978-981-08-7615-9-TE02
M3 - 会议稿件
AN - SCOPUS:79952501773
SN - 9789810876159
T3 - ISOB 2011 - Proceedings of the 1st International Symposium ISOB 2011 - Proceedings of the 1st International Symposium on Bioengineering
SP - 282
EP - 289
BT - ISOB 2011 - Proceedings of the 1st International Symposium on Bioengineering
T2 - 1st International Symposium on Bioengineering, ISOB 2011
Y2 - 19 January 2011 through 19 January 2011
ER -