Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules

Yu Yu; Stephanie Gaillard; Jude M. Phillip; Tai Chung Huang; Sneha M. Pinto; Nayara G. Tessarollo; Zhen Zhang; Akhilesh Pandey; Denis Wirtz; Ayse Ayhan; Ben Davidson; Tian Li Wang; Ie Ming Shih

doi:10.1016/j.ccell.2015.05.009

Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules

Yu Yu, Stephanie Gaillard, Jude M. Phillip, Tai Chung Huang, Sneha M. Pinto, Nayara G. Tessarollo, Zhen Zhang, Akhilesh Pandey, Denis Wirtz, Ayse Ayhan, Ben Davidson, Tian Li Wang^*, Ie Ming Shih

^*此作品的通信作者

研究成果: 期刊稿件 › 文章 › 同行評審

111 引文斯高帕斯（Scopus）

摘要

Resistance to chemotherapy represents a major obstacle for long-term remission, and effective strategies toovercome drug resistance would have significant clinical impact. We report that recurrent ovarian carcinomas after paclitaxel/carboplatin treatment have higher levels of spleen tyrosine kinase (SYK) and phospho-SYK. Invitro, paclitaxel-resistant cells expressed higher SYK, and the ratio of phospho-SYK/SYK positively associated with paclitaxel resistance in ovarian cancer cells. Inactivation of SYK by inhibitors or gene knockdown sensitized paclitaxel cytotoxicity invitro and invivo. Analysis of the phosphotyrosine proteome in paclitaxel-resistant tumor cells revealed that SYK phosphorylates tubulins and microtubule-associated proteins. Inhibition of SYK enhanced microtubule stability in paclitaxel-resistant tumor cells that were otherwise insensitive. Thus, targeting SYK pathway is a promising strategy to enhance paclitaxel response.

原文	英語
頁（從 - 到）	82-96
頁數	15
期刊	Cancer Cell
卷	28
發行號	1
DOIs	https://doi.org/10.1016/j.ccell.2015.05.009
出版狀態	已出版 - 13 07 2015
對外發佈	是

文獻附註

Publisher Copyright:
© 2015 Elsevier Inc..

UN SDG

此研究成果有助於以下永續發展目標

存取文件

10.1016/j.ccell.2015.05.009

其它文件與鏈接

Link to publication in Scopus

引用此

Yu, Y., Gaillard, S., Phillip, J. M., Huang, T. C., Pinto, S. M., Tessarollo, N. G., Zhang, Z., Pandey, A., Wirtz, D., Ayhan, A., Davidson, B., Wang, T. L., & Shih, I. M. (2015). Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules. Cancer Cell, 28(1), 82-96. https://doi.org/10.1016/j.ccell.2015.05.009

@article{621c8ae6ceb3492684a4c213ba68583a,

title = "Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules",

abstract = "Resistance to chemotherapy represents a major obstacle for long-term remission, and effective strategies toovercome drug resistance would have significant clinical impact. We report that recurrent ovarian carcinomas after paclitaxel/carboplatin treatment have higher levels of spleen tyrosine kinase (SYK) and phospho-SYK. Invitro, paclitaxel-resistant cells expressed higher SYK, and the ratio of phospho-SYK/SYK positively associated with paclitaxel resistance in ovarian cancer cells. Inactivation of SYK by inhibitors or gene knockdown sensitized paclitaxel cytotoxicity invitro and invivo. Analysis of the phosphotyrosine proteome in paclitaxel-resistant tumor cells revealed that SYK phosphorylates tubulins and microtubule-associated proteins. Inhibition of SYK enhanced microtubule stability in paclitaxel-resistant tumor cells that were otherwise insensitive. Thus, targeting SYK pathway is a promising strategy to enhance paclitaxel response.",

author = "Yu Yu and Stephanie Gaillard and Phillip, {Jude M.} and Huang, {Tai Chung} and Pinto, {Sneha M.} and Tessarollo, {Nayara G.} and Zhen Zhang and Akhilesh Pandey and Denis Wirtz and Ayse Ayhan and Ben Davidson and Wang, {Tian Li} and Shih, {Ie Ming}",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Inc..",

year = "2015",

month = jul,

day = "13",

doi = "10.1016/j.ccell.2015.05.009",

language = "英语",

volume = "28",

pages = "82--96",

journal = "Cancer Cell",

issn = "1535-6108",

publisher = "Cell Press",

number = "1",

}

Yu, Y, Gaillard, S, Phillip, JM, Huang, TC, Pinto, SM, Tessarollo, NG, Zhang, Z, Pandey, A, Wirtz, D, Ayhan, A, Davidson, B, Wang, TL & Shih, IM 2015, 'Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules', Cancer Cell, 卷 28, 編號 1, 頁 82-96. https://doi.org/10.1016/j.ccell.2015.05.009

TY - JOUR

T1 - Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules

AU - Yu, Yu

AU - Gaillard, Stephanie

AU - Phillip, Jude M.

AU - Huang, Tai Chung

AU - Pinto, Sneha M.

AU - Tessarollo, Nayara G.

AU - Zhang, Zhen

AU - Pandey, Akhilesh

AU - Wirtz, Denis

AU - Ayhan, Ayse

AU - Davidson, Ben

AU - Wang, Tian Li

AU - Shih, Ie Ming

PY - 2015/7/13

Y1 - 2015/7/13

N2 - Resistance to chemotherapy represents a major obstacle for long-term remission, and effective strategies toovercome drug resistance would have significant clinical impact. We report that recurrent ovarian carcinomas after paclitaxel/carboplatin treatment have higher levels of spleen tyrosine kinase (SYK) and phospho-SYK. Invitro, paclitaxel-resistant cells expressed higher SYK, and the ratio of phospho-SYK/SYK positively associated with paclitaxel resistance in ovarian cancer cells. Inactivation of SYK by inhibitors or gene knockdown sensitized paclitaxel cytotoxicity invitro and invivo. Analysis of the phosphotyrosine proteome in paclitaxel-resistant tumor cells revealed that SYK phosphorylates tubulins and microtubule-associated proteins. Inhibition of SYK enhanced microtubule stability in paclitaxel-resistant tumor cells that were otherwise insensitive. Thus, targeting SYK pathway is a promising strategy to enhance paclitaxel response.

AB - Resistance to chemotherapy represents a major obstacle for long-term remission, and effective strategies toovercome drug resistance would have significant clinical impact. We report that recurrent ovarian carcinomas after paclitaxel/carboplatin treatment have higher levels of spleen tyrosine kinase (SYK) and phospho-SYK. Invitro, paclitaxel-resistant cells expressed higher SYK, and the ratio of phospho-SYK/SYK positively associated with paclitaxel resistance in ovarian cancer cells. Inactivation of SYK by inhibitors or gene knockdown sensitized paclitaxel cytotoxicity invitro and invivo. Analysis of the phosphotyrosine proteome in paclitaxel-resistant tumor cells revealed that SYK phosphorylates tubulins and microtubule-associated proteins. Inhibition of SYK enhanced microtubule stability in paclitaxel-resistant tumor cells that were otherwise insensitive. Thus, targeting SYK pathway is a promising strategy to enhance paclitaxel response.

UR - http://www.scopus.com/inward/record.url?scp=84937441218&partnerID=8YFLogxK

U2 - 10.1016/j.ccell.2015.05.009

DO - 10.1016/j.ccell.2015.05.009

M3 - 文章

C2 - 26096845

AN - SCOPUS:84937441218

SN - 1535-6108

VL - 28

SP - 82

EP - 96

JO - Cancer Cell

JF - Cancer Cell

IS - 1

ER -

Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules

摘要

文獻附註

UN SDG

存取文件

其它文件與鏈接

指紋

引用此