Intracellular ATP is required for actinomycin D-induced apoptotic cell death in HeLa cells

Chih Chung Chou, Chun Yuen Lam, Benjamin Yat Ming Yung*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

19 引文 斯高帕斯(Scopus)

摘要

In the present report, we demonstrate that reduction of cellular ATP content with antimycin A blocks actinomycin D-induced apoptotic cell death in HeLa cells. Compared to cells (~80%) treated with actinomycin D (1 μg/ml, 48 h) alone in glucose-containing medium, a much smaller percentage of cells (~20%) treated with actinomycin D in the presence of antimycin A in glucose-free medium shows morphological characteristic of apoptosis. ATP-depleted cells with or without actinomycin D treatment, on the other hand, die necrotically. In cells under actinomycin D short exposure treatment (1 μg/ml; 1 h), apoptosis occurs when cellular ATP content is rapidly recovered after the removal of antimycin A and resupplementation of glucose-containing medium. In the incubation of isolated Triton-permeabilized cells with ATP (>0.5 mM), apoptotic nuclei become abundant 4 h after ATP treatment. These results implicate the requirement of ATP for the induction of apoptosis.

原文英語
頁(從 - 到)181-187
頁數7
期刊Cancer Letters
96
發行號2
DOIs
出版狀態已出版 - 25 09 1995

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