Long noncoding RNA LncHIFCAR/MIR31HG is a HIF-1α co-activator driving oral cancer progression

Jing Wen Shih*, Wei Fan Chiang, Alexander T.H. Wu, Ming Heng Wu, Ling Yu Wang, Yen Ling Yu, Yu Wen Hung, Wen Chang Wang, Cheng Ying Chu, Chiu Lien Hung, Chun A. Changou, Yun Yen, Hsing Jien Kung

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

160 引文 斯高帕斯(Scopus)

摘要

Long noncoding RNAs (lncRNAs) have been implicated in hypoxia/HIF-1-associated cancer progression through largely unknown mechanisms. Here we identify MIR31HG as a hypoxia-inducible lncRNA and therefore we name it LncHIFCAR (long noncoding HIF-1α co-activating RNA); we describe its oncogenic role as a HIF-1α co-activator that regulates the HIF-1 transcriptional network, crucial for cancer development. Extensive analyses of clinical data indicate LncHIFCAR level is substantially upregulated in oral carcinoma, significantly associated with poor clinical outcomes and representing an independent prognostic predictor. Overexpression of LncHIFCAR induces pseudo-hypoxic gene signature, whereas knockdown of LncHIFCAR impairs the hypoxia-induced HIF-1α transactivation, sphere-forming ability, metabolic shift and metastatic potential in vitro and in vivo. Mechanistically, LncHIFCAR forms a complex with HIF-1α via direct binding and facilitates the recruitment of HIF-1α and p300 cofactor to the target promoters. Our results uncover an lncRNA-mediated mechanism for HIF-1 activation and establish the clinical values of LncHIFCAR in prognosis and potential therapeutic strategy for oral carcinoma.

原文英語
文章編號15874
期刊Nature Communications
8
DOIs
出版狀態已出版 - 22 06 2017
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© 2017 The Author(s).

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