Mice lacking connective tissue growth factor in the forebrain exhibit delayed seizure response, reduced c-fos expression and different microglial phenotype following acute ptz injection

Pei Fen Siow, Chih Yu Tsao, Ho Ching Chang, Chwen Yu Chen, I. Shing Yu, Kuang Yung Lee*, Li Jen Lee*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

8 引文 斯高帕斯(Scopus)

摘要

Connective tissue growth factor (CTGF) plays important roles in the development and regeneration of the connective tissue, yet its function in the nervous system is still not clear. CTGF is expressed in some distinct regions of the brain, including the dorsal endopiriform nucleus (DEPN) which has been recognized as an epileptogenic zone. We generated a forebrain-specific Ctgf knockout (FbCtgf KO) mouse line in which the expression of Ctgf in the DEPN is eliminated. In this study, we adopted a pentylenetetrazole (PTZ)-induced seizure model and found similar severity and latencies to death between FbCtgf KO and WT mice. Interestingly, there was a delay in the seizure reactions in the mutant mice. We further observed reduced c-fos expression subsequent to PTZ treatment in the KO mice, especially in the hippocampus. While the densities of astrocytes and microglia in the hippocampus were kept constant after acute PTZ treatment, microglial morphology was different between genotypes. Our present study demonstrated that in the FbCtgf KO mice, PTZ failed to increase neuronal activity and microglial response in the hippocampus. Our results suggested that inhibition of Ctgf function may have a therapeutic potential in preventing the pathophysiology of epilepsy.

原文英語
文章編號4921
頁(從 - 到)1-14
頁數14
期刊International Journal of Molecular Sciences
21
發行號14
DOIs
出版狀態已出版 - 02 07 2020

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© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

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