Microencapsulation of islets in PEG-amine modified alginate-poly(L- lysine)-alginate microcapsules for constructing bioartificial pancreas

Jyh Ping Chen*, I. Ming Chu, Meng Yi Shiao, Brend Ray Sea Hsu, Shin Huei Fu

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

46 引文 斯高帕斯(Scopus)

摘要

Two positively charged derivatives of poly(ethylene glycol) (PEG) were coated onto alginate-poly(L-lysine)-alginate (A-P-A) microcapsule by allowing them to interact them with the negatively charged alginate on the capsule surface. The polymers are methoxypolyoxyethylene amine (PEGA1) and polyoxyethylene bis(amine) (PEGA2), which contain charged amine groups at one or both ends, respectively, with PEG as the backbone. The coating of the microcapsules with PEG-amine resulted in a much smoother capsule surface than A-P-A microcapsule surfaces as examined under a scanning electron microscope. The diffusivity of bovine serum albumin into the microcapsules remained the same after PEGA1 coating. But the diffusivity decreased to less than one-fifth that in A-P-A microcapsules coated with PEGA2. The biocompatibility of the microcapsules also improved as investigated by an in vivo study. Microcapsules were implanted in the peritoneal cavity of BALB/c mice and retrieved 120 d after implantation. The fibrotic action against A-P-A microcapsules was severe and the capsules retrieved by peritoneal lavage aggregated into clusters. In contrast, the surface-modified capsules were free-flowing and free of cell overgrowth. Secretion of insulin from rat islets within A-P-A-PEGA microcapsules responded well to changes in glucose concentration in a static glucose test. Intraperitoneal transplantation of the microencapsulated islets into streptozotocin-induced diabetic mice could maintain normal blood glucose levels in test animals for up to 200 d without immunosuppression.

原文英語
頁(從 - 到)185-190
頁數6
期刊Journal of Fermentation and Bioengineering
86
發行號2
DOIs
出版狀態已出版 - 1998

指紋

深入研究「Microencapsulation of islets in PEG-amine modified alginate-poly(L- lysine)-alginate microcapsules for constructing bioartificial pancreas」主題。共同形成了獨特的指紋。

引用此