摘要
A novel optical heterodyne surface plasmon resonance (SPR) biosensor using Zeeman laser is proposed. There are two surface plasma waves (SPWs) being excited by two correlated P polarized waves in an SPR device of Kretschmann configuration. The two reflected P waves are optically heterodyned so that the magnitude of the heterodyned signal is proportional to the multiplication of two attenuated reflected P waves. Then the detection sensitivity and the dynamical range based on this amplitude sensitive method are enhanced. In the experiment, the kinetics between mouse IgG and anti-mouse IgG is obtained according to the sensograms of different concentrations of anti-mouse IgG. The detection sensitivity corresponding to 0.2 nM is achieved. In addition, a concentration of 5 ng/ml of protein G interacting with mouse IgG is measured successfully.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 149-156 |
| 頁數 | 8 |
| 期刊 | Proceedings of SPIE - The International Society for Optical Engineering |
| 卷 | 4957 |
| DOIs | |
| 出版狀態 | 已出版 - 2003 |
| 對外發佈 | 是 |
| 事件 | PROGRESS IN BIOMEDICAL OPTICS AND IMAGING: Optical Fibers and Sensors for Medical Applications III - San Jose, CA, 美國 持續時間: 25 01 2003 → 28 01 2003 |