Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

Wen Chang Chang; Muh Lin Lee; Chen Kung Chou; Sheng Chung Lee

doi:10.1016/0003-2697(83)90018-0

Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

Wen Chang Chang, Muh Lin Lee, Chen Kung Chou, Sheng Chung Lee^*

^*此作品的通信作者

研究成果: 期刊稿件 › 文章 › 同行評審

9 引文斯高帕斯（Scopus）

摘要

One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with R_f = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at R_f = 0.72.

原文	英語
頁（從 - 到）	342-344
頁數	3
期刊	Analytical Biochemistry
卷	132
發行號	2
DOIs	https://doi.org/10.1016/0003-2697(83)90018-0
出版狀態	已出版 - 15 07 1983
對外發佈	是

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title = "Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine",

abstract = "One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.",

keywords = "TLC, phosphoamino acids, polyamide",

author = "Chang, {Wen Chang} and Lee, {Muh Lin} and Chou, {Chen Kung} and Lee, {Sheng Chung}",

year = "1983",

month = jul,

day = "15",

doi = "10.1016/0003-2697(83)90018-0",

language = "英语",

volume = "132",

pages = "342--344",

journal = "Analytical Biochemistry",

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T1 - Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

AU - Chang, Wen Chang

AU - Lee, Muh Lin

AU - Chou, Chen Kung

AU - Lee, Sheng Chung

PY - 1983/7/15

Y1 - 1983/7/15

N2 - One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.

AB - One-dimensional thin-layer chromatography on polyamide plates offers an easy and rapid identification of O-phosphotyrosine. The thin-layer plate is developed for 30 min in 5% propionic acid containing 0.013%-0.025% sodium dodecyl sulfate. O-Phosphotyrosine, with Rf = 0.54, can be well separated from O-phosphothreonine and O-phosphoserine, which comigrate at Rf = 0.72.

KW - TLC

KW - phosphoamino acids

KW - polyamide

UR - http://www.scopus.com/inward/record.url?scp=0020517188&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(83)90018-0

DO - 10.1016/0003-2697(83)90018-0

M3 - 文章

C2 - 6194716

AN - SCOPUS:0020517188

SN - 0003-2697

VL - 132

SP - 342

EP - 344

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -

Polyamide thin-layer chromatography of phosphorylated tyrosine, threonine, and serine

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