Polyvalency of Tn (GalNAcα1→Ser/Thr) glycotope as a critical factor for Vicia villosa B4 and glycoprotein interactions

Albert M. Wu*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

38 引文 斯高帕斯(Scopus)

摘要

Vicia villosa B4 (VVL-B4) is an important lectin for detecting exposed Tn (GalNAcα1-Ser/Thr) determinants on cancer cells. In order to elucidate the binding factors involved in VVL-B4 and glycotope interaction, the binding properties of this lectin were analyzed by enzyme-linked lectinosorbent and inhibition assays. From the results, it is concluded that the most critical factor affecting VVL-B4 binding is polyvalency at the α anomer of Gal with -NH CH3CO at carbon-2 (Tn epitope), which enhances the reactivity by 3.3×105 times over monovalent Gal. The reactivities of glycotopes can be ranked as follows: high density Tn cluster≫Tn glycopeptides (MW<3.0×10 3)≫monomeric Tn to tri- Tn glycopeptides⋙other GalNAcα/β-related structural units>Gal and Galα- or β-linked ligands, demonstrating the essential role of the polyvalency of Tn glycotopes in the enhancement of the binding.

原文英語
頁(從 - 到)51-58
頁數8
期刊FEBS Letters
562
發行號1-3
DOIs
出版狀態已出版 - 26 03 2004

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