Proteomic analysis of the effects of baicalein on colorectal cancer cells

Wen Shih Huang, Yi Hung Kuo, Chih Chien Chin, Jeng Yi Wang, Hong Ren Yu, Jiunn Ming Sheen, Shui Yi Tung, Chien Heng Shen, Te Chuan Chen, Mei Lan Sung, Hwey Fang Liang, Hsing Chun Kuo*

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

46 引文 斯高帕斯(Scopus)

摘要

Baicalein is the flavonoids with multiple pharmacological activities. The aim of our study was to investigate the effects of baicalein on colorectal cancer (CRC) and to recognize the targets of baicalein treatment. To better understand baicalein's target, proteomic approaches were used to purify and identify the protein substrates using 2D difference gel electrophoresis (2D SDS-PAGE) to elucidate proteins differential display. Results from this study investigate that baicalein treatment of CRC cells results in reduced cell proliferation. As a result, differential protein displays between baicalein-treated and untreated CRC were determined and validated. There were 11 differentially expressed proteins between baicalein-treated and untreated CRC. Furthermore, we demonstrate that baicalein inhibits cancer cell proliferation and reduced reactive oxygen species (ROS) by up-regulating the levels of peroxiredoxin-6 (PRDX6). Knockdown of PRDX6 in baicalein-treated CRC cells by specific small interfering RNA resulted in ROS production and proliferation, opposite of the baicalein treatment scenario as indicated by cell cycle distribution. These results illustrate that baicalein up-regulates the expression of PRDX6, which attenuates the generation of ROS and inhibits the growth of CRC cells, whereas baicalein treatment have no effect on normal epithelial cells.

原文英語
頁(從 - 到)810-819
頁數10
期刊Proteomics
12
發行號6
DOIs
出版狀態已出版 - 03 2012

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