Rapid identification of Mycobacterium tuberculosis infection by a new array format-based surface plasmon resonance method

Shang Chen Hsieh, Chia Chen Chang, Chia Chen Lu, Chia Fong Wei, Chuan Sheng Lin, Hsin Chih Lai*, Chii Wann Lin

*此作品的通信作者

研究成果: 期刊稿件文章同行評審

40 引文 斯高帕斯(Scopus)

摘要

Tubercle bacillus [TB] is one of the most important chronic infectious diseases that cause millions of deaths annually. While conventional smear microscopy and culture methods are widely used for diagnosis of TB, the former is insensitive, and the latter takes up to 6 to 8 weeks to provide a result, limiting the value of these methods in aiding diagnosis and intermediate decisions on treatment. Therefore, a rapid detection method is essential for the diagnosis, prognosis assessment, and recurrence monitoring. A new surface plasmon resonance [SPR] biosensor based on an array format, which allowed immobilizing nine TB antigens onto the sensor chip, was constructed. Simultaneous determination of multiple TB antibodies in serum had been accomplished with this array-based SPR system. The results were compared with enzyme-linked immunosorbent assay, a conventional immunological method. Array-based SPR showed more advantages in providing label-free and real-time detection. Additionally, the high sensitivity and specificity for the detection of TB infection showed its potential for future development of biosensor arrays for TB diagnosis.

原文英語
文章編號180
頁(從 - 到)1-6
頁數6
期刊Nanoscale Research Letters
7
DOIs
出版狀態已出版 - 2012

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