Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

Han Wen Guo; Chien Tsun Chen; Yau Huei Wei; Oscar K. Lee; Vladimir Gukassyan; Fu Jen Kao; Hsing Wen Wang

doi:10.1117/1.2990752

Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

Han Wen Guo, Chien Tsun Chen, Yau Huei Wei, Oscar K. Lee, Vladimir Gukassyan, Fu Jen Kao, Hsing Wen Wang^*

^*此作品的通信作者

National Yang Ming Chiao Tung University

研究成果: 期刊稿件 › 文章 › 同行評審

55 引文斯高帕斯（Scopus）

摘要

The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.

原文	英語
文章編號	050505
期刊	Journal of Biomedical Optics
卷	13
發行號	5
DOIs	https://doi.org/10.1117/1.2990752
出版狀態	已出版 - 2008
對外發佈	是

存取文件

10.1117/1.2990752

其它文件與鏈接

Link to publication in Scopus

引用此

@article{37e155806c1148f3851654dc9e0a149c,

title = "Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies",

abstract = "The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.",

keywords = "fluorescence lifetime, microscopy, stem cell",

author = "Guo, {Han Wen} and Chen, {Chien Tsun} and Wei, {Yau Huei} and Lee, {Oscar K.} and Vladimir Gukassyan and Kao, {Fu Jen} and Wang, {Hsing Wen}",

year = "2008",

doi = "10.1117/1.2990752",

language = "英语",

volume = "13",

journal = "Journal of Biomedical Optics",

issn = "1083-3668",

publisher = "SPIE",

number = "5",

}

TY - JOUR

T1 - Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

AU - Guo, Han Wen

AU - Chen, Chien Tsun

AU - Wei, Yau Huei

AU - Lee, Oscar K.

AU - Gukassyan, Vladimir

AU - Kao, Fu Jen

AU - Wang, Hsing Wen

PY - 2008

Y1 - 2008

N2 - The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.

AB - The metabolic changes of human mesenchymal stem cells (hMSCs) during osteogenic differentiation were accessed by reduced nicotinamide adenine dinucleotide (NADH) fluorescence lifetime. An increase in mean fluorescence lifetime and decrease in the ratio between free NADH and protein-bound NADH correlated with our previously reported increase in the adenosine triphosphate (ATP) level of hMSCs during differentiation. These findings suggest that NADH fluorescence lifetime may serve as a new optical biomarker for noninvasive selection of stem cells from differentiated progenies.

KW - fluorescence lifetime

KW - microscopy

KW - stem cell

UR - http://www.scopus.com/inward/record.url?scp=60849084594&partnerID=8YFLogxK

U2 - 10.1117/1.2990752

DO - 10.1117/1.2990752

M3 - 文章

C2 - 19021377

AN - SCOPUS:60849084594

SN - 1083-3668

VL - 13

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 5

M1 - 050505

ER -

Reduced nicotinamide adenine dinucleotide fluorescence lifetime separates human mesenchymal stem cells from differentiated progenies

摘要

存取文件

其它文件與鏈接

指紋

引用此