Repellency of essential oils of Cryptomeria japonica (Pinaceae) against adults of the mosquitoes Aedes aegypti and Aedes albopictus (Diptera:Culicidae)

The purpose of this study was to investigate the repellent activities of essential oils from Cryptomeria japonica (sugi) against adults of mosquitoes Aedes aegypti and Aedes albopictus. Comparison of essential oils from four different plant parts of C. japonica revealed that essential oil from its leaf exhibited the best repellent activity against mosquitoes. To understand the relationship between volatile organic compounds and repellent activity, the solid-phase microextraction (SPME) method was employed to analyze volatile organic compounds of leaf essential oil. The SPME fiber was coated with divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS). The major volatile organic compounds in the cage were 3-carene, α-terpinene, limonene, γ-terpinene, and terpinolene at 0 min. Results demonstrated that (-)-terpinen-4-ol was the major volatile organic compound adsorbed by SPME fiber during repellent assays. Furthermore, the repellent activities of six compounds against adults of the mosquitoes were evaluated, and the results revealed that (-)-terplnen-4-ol exhibited the best repellent activity against A. aegypti and A. albopictus.