Selective suppression of insulin-induced proliferation of cultured human hepatoma cells by somatostatin

C. K. Chou, L. T. Ho, L. P. Ting, C. P. Hu, T. S. Su, W. C. Chang, C. S. Suen, M. Y. Huang, C. M. Chang

研究成果: 期刊稿件文章同行評審

48 引文 斯高帕斯(Scopus)

摘要

The effects of somatostatin (SRIF), insulin, and triiodothyronine (T3) on the growth of human hepatoma cells were investigated on the well-differentiated human hepatoma cell line Hep3B. Results showed that both insulin and T3 can stimulate cell growth of serum starved Hep3B cells at physiological concentrations. SRIF alone showed little growth-promoting activity. When added concurrently with insulin, however, SRIF suppressed the insulin-induced cell proliferation in a dose-dependent manner. On the other hand, SRIF had no inhibitory effect on T3-induced cell proliferation. SRIF is labile in the medium, with a half-life of about 2 h during culture incubation. SRIF did not disturb the insulin binding to its surface receptors nor inhibit the insulin-dependent receptor kinase activity of Hep3B cells in vitro. These results suggest that postreceptor regulation may be involved. The selective suppression by SRIF of insulin-induced cell growth provides an unique approach to the study of insulin actions on proliferation of human hepatoma cells.

原文英語
頁(從 - 到)175-178
頁數4
期刊Journal of Clinical Investigation
79
發行號1
DOIs
出版狀態已出版 - 1987
對外發佈

指紋

深入研究「Selective suppression of insulin-induced proliferation of cultured human hepatoma cells by somatostatin」主題。共同形成了獨特的指紋。

引用此