Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system

Pei Yin Chi; Ting Wei Chuang; Tzu Tsai Chu; Chia Tzu Kuo; Yu Ting Wu; Vahid Farmehini; Dar Bin Shieh; Fan Gang Tseng; Yau Huei Wei; Nathan Swami; Chia Fu Chou

Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system

Pei Yin Chi, Ting Wei Chuang, Tzu Tsai Chu, Chia Tzu Kuo, Yu Ting Wu, Vahid Farmehini, Dar Bin Shieh, Fan Gang Tseng, Yau Huei Wei, Nathan Swami, Chia Fu Chou^*

^*此作品的通信作者

研究成果: 圖書/報告稿件的類型 › 會議稿件 › 同行評審

摘要

It is imperative to develop tools to characterize cells with the diseased and normal mitochondria to monitor the healthiness of cells and take preventive measures for potential mitochondrial disease development. Here, we adopt insulator dielectrophoresis (i-DEP), by utilizing the intrinsic dielectric response of a cell to an external AC electric field to differentiate cells, containing partially diseased and normal mitochondria. Taking advantage of dielectrophoresis, DEP, principle, we developed a quick diagnostic method to detect mitochondrial disease in living cells, which is not readily achievable by conventional cell cytometry.

原文	英語
主出版物標題	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
發行者	Chemical and Biological Microsystems Society
頁面	1372-1373
頁數	2
ISBN（電子）	9781733419000
出版狀態	已出版 - 2019
對外發佈	是
事件	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, 瑞士持續時間: 27 10 2019 → 31 10 2019

出版系列

名字	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Conference

Conference	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
國家/地區	瑞士
城市	Basel
期間	27/10/19 → 31/10/19

文獻附註

Publisher Copyright:
© 2019 CBMS-0001.

其它文件與鏈接

Link to publication in Scopus

引用此

Chi, P. Y., Chuang, T. W., Chu, T. T., Kuo, C. T., Wu, Y. T., Farmehini, V., Shieh, D. B., Tseng, F. G., Wei, Y. H., Swami, N., & Chou, C. F. (2019). Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system. 於 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 (頁 1372-1373). (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019). Chemical and Biological Microsystems Society.

Chi, Pei Yin ; Chuang, Ting Wei ; Chu, Tzu Tsai 等. / Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, 2019. 頁 1372-1373 (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019).

@inproceedings{b7dc43d1c72d46459e17f3c54e9f1dbd,

title = "Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system",

abstract = "It is imperative to develop tools to characterize cells with the diseased and normal mitochondria to monitor the healthiness of cells and take preventive measures for potential mitochondrial disease development. Here, we adopt insulator dielectrophoresis (i-DEP), by utilizing the intrinsic dielectric response of a cell to an external AC electric field to differentiate cells, containing partially diseased and normal mitochondria. Taking advantage of dielectrophoresis, DEP, principle, we developed a quick diagnostic method to detect mitochondrial disease in living cells, which is not readily achievable by conventional cell cytometry.",

keywords = "Chip, Diagnosis, Dielectrophoresis (DEP), Microfluidic, Mitochondria",

author = "Chi, {Pei Yin} and Chuang, {Ting Wei} and Chu, {Tzu Tsai} and Kuo, {Chia Tzu} and Wu, {Yu Ting} and Vahid Farmehini and Shieh, {Dar Bin} and Tseng, {Fan Gang} and Wei, {Yau Huei} and Nathan Swami and Chou, {Chia Fu}",

note = "Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

year = "2019",

language = "英语",

series = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

publisher = "Chemical and Biological Microsystems Society",

pages = "1372--1373",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

}

Chi, PY, Chuang, TW, Chu, TT, Kuo, CT, Wu, YT, Farmehini, V, Shieh, DB, Tseng, FG, Wei, YH, Swami, N & Chou, CF 2019, Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system. 於 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, Chemical and Biological Microsystems Society, 頁 1372-1373, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, Basel, 瑞士, 27/10/19.

Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system. / Chi, Pei Yin; Chuang, Ting Wei; Chu, Tzu Tsai 等.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society, 2019. p. 1372-1373 (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019).

研究成果: 圖書/報告稿件的類型 › 會議稿件 › 同行評審

TY - GEN

T1 - Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system

AU - Chi, Pei Yin

AU - Chuang, Ting Wei

AU - Chu, Tzu Tsai

AU - Kuo, Chia Tzu

AU - Wu, Yu Ting

AU - Farmehini, Vahid

AU - Shieh, Dar Bin

AU - Tseng, Fan Gang

AU - Wei, Yau Huei

AU - Swami, Nathan

AU - Chou, Chia Fu

PY - 2019

Y1 - 2019

N2 - It is imperative to develop tools to characterize cells with the diseased and normal mitochondria to monitor the healthiness of cells and take preventive measures for potential mitochondrial disease development. Here, we adopt insulator dielectrophoresis (i-DEP), by utilizing the intrinsic dielectric response of a cell to an external AC electric field to differentiate cells, containing partially diseased and normal mitochondria. Taking advantage of dielectrophoresis, DEP, principle, we developed a quick diagnostic method to detect mitochondrial disease in living cells, which is not readily achievable by conventional cell cytometry.

AB - It is imperative to develop tools to characterize cells with the diseased and normal mitochondria to monitor the healthiness of cells and take preventive measures for potential mitochondrial disease development. Here, we adopt insulator dielectrophoresis (i-DEP), by utilizing the intrinsic dielectric response of a cell to an external AC electric field to differentiate cells, containing partially diseased and normal mitochondria. Taking advantage of dielectrophoresis, DEP, principle, we developed a quick diagnostic method to detect mitochondrial disease in living cells, which is not readily achievable by conventional cell cytometry.

KW - Chip

KW - Diagnosis

KW - Dielectrophoresis (DEP)

KW - Microfluidic

KW - Mitochondria

UR - http://www.scopus.com/inward/record.url?scp=85094954307&partnerID=8YFLogxK

M3 - 会议稿件

AN - SCOPUS:85094954307

T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

SP - 1372

EP - 1373

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

PB - Chemical and Biological Microsystems Society

T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Y2 - 27 October 2019 through 31 October 2019

ER -

Chi PY, Chuang TW, Chu TT, Kuo CT, Wu YT, Farmehini V 等. Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system. 於 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. Chemical and Biological Microsystems Society. 2019. p. 1372-1373. (23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019).

Separation of mitochondrial diseased cells based on organelle-level difference using a dep microfluidic system

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出版系列

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