Soluble Dectin-1 as a tool to detect β-glucans

Lisa M. Graham; S. Vicky Tsoni; Janet A. Willment; David L. Williams; Philip R. Taylor; Siamon Gordon; Kevin Dennehy; Gordon D. Brown

doi:10.1016/j.jim.2006.05.013

Soluble Dectin-1 as a tool to detect β-glucans

Lisa M. Graham, S. Vicky Tsoni, Janet A. Willment, David L. Williams, Philip R. Taylor, Siamon Gordon, Kevin Dennehy, Gordon D. Brown^*

^*此作品的通信作者

研究成果: 期刊稿件 › 文章 › 同行評審

105 引文斯高帕斯（Scopus）

摘要

β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

原文	英語
頁（從 - 到）	164-169
頁數	6
期刊	Journal of Immunological Methods
卷	314
發行號	1-2
DOIs	https://doi.org/10.1016/j.jim.2006.05.013
出版狀態	已出版 - 31 07 2006
對外發佈	是

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title = "Soluble Dectin-1 as a tool to detect β-glucans",

abstract = "β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.",

keywords = "Beta-glucan, C-type lectin, Dectin-1, ELISA, Innate immunity",

author = "Graham, {Lisa M.} and Tsoni, {S. Vicky} and Willment, {Janet A.} and Williams, {David L.} and Taylor, {Philip R.} and Siamon Gordon and Kevin Dennehy and Brown, {Gordon D.}",

year = "2006",

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T1 - Soluble Dectin-1 as a tool to detect β-glucans

AU - Graham, Lisa M.

AU - Tsoni, S. Vicky

AU - Willment, Janet A.

AU - Williams, David L.

AU - Taylor, Philip R.

AU - Gordon, Siamon

AU - Dennehy, Kevin

AU - Brown, Gordon D.

PY - 2006/7/31

Y1 - 2006/7/31

N2 - β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

AB - β-Glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian β-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble β-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

KW - Beta-glucan

KW - C-type lectin

KW - Dectin-1

KW - ELISA

KW - Innate immunity

UR - http://www.scopus.com/inward/record.url?scp=33747047848&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2006.05.013

DO - 10.1016/j.jim.2006.05.013

M3 - 文章

C2 - 16844139

AN - SCOPUS:33747047848

SN - 0022-1759

VL - 314

SP - 164

EP - 169

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Soluble Dectin-1 as a tool to detect β-glucans

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